B6Brd;B6N-Tyr^c-Brd Zfp106^{tm1a(KOMP)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:10852
Citation information	RRID:IMSR_EM:10852 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6Brd;B6N-Tyr^c-Brd Zfp106^{tm1a(KOMP)Wtsi}/WtsiH
Alternative name	EPD0033_4_C03
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Zfp106^{tm1a(KOMP)Wtsi}
Gene/Transgene symbol	Zfp106
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0033_4_C03. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	heterozygous males
Breeding at archiving centre	Maintained on a C57BL/6NTac background

Disease and phenotype information

IMPC phenotypes (allele matching)

increased circulating calcium level / IMPC
decreased body weight / IMPC
abnormal gait / IMPC
abnormal posture / IMPC
increased thermal nociceptive threshold / IMPC
abnormal external female genitalia morphology / IMPC

IMPC phenotypes (gene matching)

increased circulating calcium level / IMPC
abnormal external female genitalia morphology / IMPC
increased thermal nociceptive threshold / IMPC
decreased body weight / IMPC
abnormal gait / IMPC
abnormal posture / IMPC

MGI phenotypes (allele matching)

increased circulating calcium level / MGI
kyphosis / MGI
tremors / MGI
motor neuron degeneration / MGI
abnormal spinal cord morphology / MGI
small dorsal root ganglion / MGI
small L4 dorsal root ganglion / MGI
small L5 dorsal root ganglion / MGI
weight loss / MGI
hypoactivity / MGI
impaired coordination / MGI
abnormal gait / MGI
limb grasping / MGI
impaired limb coordination / MGI
premature death / MGI
abnormal muscle physiology / MGI
gliosis / MGI
neurodegeneration / MGI
chromatolysis / MGI
abnormal soleus morphology / MGI
abnormal gastrocnemius morphology / MGI
abnormal redox activity / MGI
astrocytosis / MGI
nervous system phenotype / MGI
abnormal muscle electrophysiology / MGI
positive geotaxis / MGI
abnormal axon morphology / MGI
axon degeneration / MGI
abnormal mitochondrial physiology / MGI
abnormal lumbar dorsal root ganglion morphology / MGI
microgliosis / MGI
increased variability of skeletal muscle fiber size / MGI
centrally nucleated skeletal muscle fibers / MGI
decreased skeletal muscle fiber number / MGI
abnormal skeletal muscle fiber type ratio / MGI
skeletal muscle fiber atrophy / MGI
skeletal muscle atrophy / MGI
skeletal muscle fibrosis / MGI
decreased gastrocnemius weight / MGI
decreased soleus weight / MGI
decreased tibialis anterior weight / MGI
decreased grip strength / MGI
abnormal mitochondrial ATP synthesis coupled electron transport / MGI
increased spinal cord apoptosis / MGI
abnormal external male genitalia morphology / MGI

MGI phenotypes (gene matching)

kyphosis / MGI
increased circulating calcium level / MGI
tremors / MGI
abnormal skeletal muscle morphology / MGI
motor neuron degeneration / MGI
decreased motor neuron number / MGI
abnormal spinal cord morphology / MGI
small dorsal root ganglion / MGI
small L4 dorsal root ganglion / MGI
small L5 dorsal root ganglion / MGI
weight loss / MGI
hypoactivity / MGI
impaired coordination / MGI
abnormal gait / MGI
limb grasping / MGI
impaired limb coordination / MGI
premature death / MGI
abnormal muscle physiology / MGI
gliosis / MGI
neurodegeneration / MGI
chromatolysis / MGI
abnormal soleus morphology / MGI
abnormal gastrocnemius morphology / MGI
abnormal redox activity / MGI
astrocytosis / MGI
nervous system phenotype / MGI
abnormal muscle electrophysiology / MGI
abnormal physical strength / MGI
cachexia / MGI
positive geotaxis / MGI
abnormal axon morphology / MGI
axon degeneration / MGI
abnormal mitochondrial physiology / MGI
abnormal lumbar dorsal root ganglion morphology / MGI
microgliosis / MGI
abnormal external male genitalia morphology / MGI
increased variability of skeletal muscle fiber size / MGI
centrally nucleated skeletal muscle fibers / MGI
decreased skeletal muscle fiber number / MGI
abnormal skeletal muscle fiber type ratio / MGI
skeletal muscle fiber degeneration / MGI
skeletal muscle fiber atrophy / MGI
skeletal muscle atrophy / MGI
skeletal muscle fibrosis / MGI
decreased gastrocnemius weight / MGI
decreased soleus weight / MGI
decreased tibialis anterior weight / MGI
decreased grip strength / MGI
abnormal mitochondrial ATP synthesis coupled electron transport / MGI
increased spinal cord apoptosis / MGI

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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B6Brd;B6N-Tyrc-Brd Zfp106tm1a(KOMP)Wtsi/WtsiH