C57BL/6N-Serf2tm1d(EUCOMM)Hmgu/H

Status

Available to order

EMMA IDEM:10739
International strain nameC57BL/6N-Serf2tm1d(EUCOMM)Hmgu/H
Alternative nameHAR0000_1_H02
Strain typeTargeted Mutant Strains : Targeted Conditional
Allele/Transgene symbolSerf2tm1d(EUCOMM)Hmgu
Gene/Transgene symbolSerf2
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider MRC, Medical Research Council
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis line originates from EUCOMM ES clone HAR0000_1_H02, after breeding with a Flp recombinase deleter line to convert the original targeted allele tm1a (knock-out first allele) into a deletion allele tm1d (post-Flp and Cre with no reporter). For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
References
  • Deletion of SERF2 in mice delays embryonic development and alters amyloid deposit structure in the brain.;Stroo Esther, Janssen Leen, Sin Olga, Hogewerf Wytse, Koster Mirjam, Harkema Liesbeth, Youssef Sameh A, Beschorner Natalie, Wolters Anouk Hg, Bakker Bjorn, Becker Lore, Garrett Lilian, Marschall Susan, Hoelter Sabine M, Wurst Wolfgang, Fuchs Helmut, Gailus-Durner Valerie, Hrabe de Angelis Martin, Thathiah Amantha, Foijer Floris, van de Sluis Bart, van Deursen Jan, Jucker Matthias, de Bruin Alain, Nollen Ellen Aa, ;2023;Life science alliance;6;; 37130781

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)
  • preweaning lethality, complete penetrance / IMPC

Literature references

  • Deletion of SERF2 in mice delays embryonic development and alters amyloid deposit structure in the brain.;Stroo Esther, Janssen Leen, Sin Olga, Hogewerf Wytse, Koster Mirjam, Harkema Liesbeth, Youssef Sameh A, Beschorner Natalie, Wolters Anouk Hg, Bakker Bjorn, Becker Lore, Garrett Lilian, Marschall Susan, Hoelter Sabine M, Wurst Wolfgang, Fuchs Helmut, Gailus-Durner Valerie, Hrabe de Angelis Martin, Thathiah Amantha, Foijer Floris, van de Sluis Bart, van Deursen Jan, Jucker Matthias, de Bruin Alain, Nollen Ellen Aa, ;2023;Life science alliance;6;; 37130781

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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