B6;129-Col15a1^tm2.1Pih Tg(Tie1-cre)9Ref/Oulu

Status	Available to order
EMMA ID	EM:10618
Citation information	RRID:IMSR_EM:10618 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6;129-Col15a1^tm2.1Pih Tg(Tie1-cre)9Ref/Oulu
Alternative name	Endothelial cell-specific collagen XV knockout in B6 background
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Col15a1^tm2.1Pih, Tg(Tie1-cre)9Ref
Gene/Transgene symbol	Col15a1, Tg(Tie1-cre)9Ref

Information from provider

Provider	Taina Pihlajaniemi
Provider affiliation	Faculty of Biochemistry and Molecular Medicine, University of Oulu
Additional owner	Tg(Tie1-cre)9Ref originates from Prof. Reinhard Fässler, Max Planck Institute of Biochemistry, Martinsried, Germany.
Genetic information	Col15a1 contains two loxP sites, one 900 bp upstream of the first exon and a second one 120 bp downstream of the second exon. Cre-mediated recombination of neo+tk selection cassette out was performed in ES cells. Col15a1 floxed mice were bred with the endothelial cell-specific cre recombinase-expressing line, Tg(Tie1-cre)9Ref.
Phenotypic information	Homozygous: No obvious phenotype Heterozygous: Not observed
Breeding history	Mice were bred 2-3 generations with C57BL/6J mice, cross-bred with Tg(Tie1-cre)9Ref in C57BL/6J background.
References	Lack of type XV collagen causes a skeletal myopathy and cardiovascular defects in mice.;Eklund L, Piuhola J, Komulainen J, Sormunen R, Ongvarrasopone C, Fássler R, Muona A, Ilves M, Ruskoaho H, Takala T E, Pihlajaniemi T, ;2001;Proceedings of the National Academy of Sciences of the United States of America;98;1194-9; 11158616 Lack of type XV collagen causes a skeletal myopathy and cardiovascular defects in mice.;Eklund L, Piuhola J, Komulainen J, Sormunen R, Ongvarrasopone C, Fássler R, Muona A, Ilves M, Ruskoaho H, Takala T E, Pihlajaniemi T, ;2001;Proceedings of the National Academy of Sciences of the United States of America;98;1194-9; 11158616 Tie-1-directed expression of Cre recombinase in endothelial cells of embryoid bodies and transgenic mice.;Gustafsson E, Brakebusch C, Hietanen K, Fässler R, ;2001;Journal of cell science;114;671-6; 11171372
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	University of Oulu, Oulu, Finland
Animals used for archiving	heterozygous C57BL/6JOlaHsd males, wild-type C57BL/6JOlaHsd females
Stage of embryos	Morula

Disease and phenotype information

MGI phenotypes (gene matching)

abnormal muscle regeneration / MGI
myopathy / MGI
abnormal cardiovascular system physiology / MGI
abnormal muscle morphology / MGI
abnormal capillary morphology / MGI
abnormal muscle fiber morphology / MGI
abnormal vascular endothelial cell morphology / MGI

Literature references

Lack of type XV collagen causes a skeletal myopathy and cardiovascular defects in mice.;Eklund L, Piuhola J, Komulainen J, Sormunen R, Ongvarrasopone C, Fássler R, Muona A, Ilves M, Ruskoaho H, Takala T E, Pihlajaniemi T, ;2001;Proceedings of the National Academy of Sciences of the United States of America;98;1194-9; 11158616
Tie-1-directed expression of Cre recombinase in endothelial cells of embryoid bodies and transgenic mice.;Gustafsson E, Brakebusch C, Hietanen K, Fässler R, ;2001;Journal of cell science;114;671-6; 11171372

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6;129-Col15a1tm2.1Pih Tg(Tie1-cre)9Ref/Oulu