B6;D2-Tg(Col13a1)1Pih/Oulu
| Status | Available to order |
| EMMA ID | EM:10594 |
| International strain name | B6;D2-Tg(Col13a1)1Pih/Oulu |
| Alternative name | Tg(Col13a1)1Pih |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Col13a1)1Pih |
| Gene/Transgene symbol | Tg(Col13a1)1Pih |
Information from provider
| Provider | Taina Pihlajaniemi |
| Provider affiliation | Faculty of Biochemistry and Molecular medicine, University of Oulu |
| Genetic information | The construct is 4,7 kb in length. It consists of the following fragments. First fragment is a 2,5 kb starting from -984 (with respect to the ATG codon) and thus containing the Col13a1 promoter, and the first protein coding exon as well as 0,8 kb of following sequences in the first intron. This is linked to the second genomic fragment containing 159 bp in 3 prime of the first intron and 91 bp of the second exon. This is further linked to a Col13a1 cDNA clone containing rest of the second exon and exons 3-41 but lacking 270 bp between exons 18 and 24. Finally, sequences coding for a termination and SV40 polyA are linked 3 prime from the cDNA clone. A base insertion was later detected at the 3 prime end of the cDNA causing the shortening of the COL3 domain by six collagenous repeats and the lack of the NC4 domain. |
| Phenotypic information | Homozygous:Transgene expression led to fetal lethality in offspring from heterozygous mating with two distinct phenotypes. The early phenotype fetuses were aborted by day 10.5 of development due to a lack of fusion of the chorionic and allantoic membranes. The late phenotype fetuses were aborted by day 13.5 of development and displayed a weak heartbeat, defects of the adherence junctions in the heart with detachment of myofilaments and abnormal staining for the adherence junction component cadherin. Decreased microvessel formation was observed in certain regions of the fetus and the placenta. Furthermore, atrioventricular valve regurgitation (AVVR) was detected in Doppler ultrasonography; histological analysis and histology showed the trabeculation of the ventricles to be reduced and the myocardium to be thinner.Heterozygous:Mice heterozygous for a transgene Col13a1(del) expressing a mutant collagen XIII developed clonal mature B-cell lineage lymphomas originating in mesenteric lymph nodes (MLN). The tumors were associated with T cells and macrophages. The incidence of disease was reduced 2-fold in transgenic mice raised under specific pathogen-free conditions, suggesting a role for infectious agents. The lymphomas did not express the mutant collagen XIII, indicating that its influence on tumorigenesis was B-cell extrinsic and likely to be associated with collagen XIII-positive tissues drained by the MLN. Studies of the small intestines of transgenic mice showed that the subepithelial basement membranes (BM) were highly abnormal and that they exhibited heightened expression of genes involved in immune responses. |
| Breeding history | The construct was microinjected into one-cell stage embryos from B6D2F1 (hybrid of C57BL/6J x DBA/2J). The line was maintained as hybrid for about a decade, after which it was frozen using C57BL/6JOlaHsd females. |
| References |
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| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Oulu, Oulu, Finland |
| Animals used for archiving | heterozygous 0, wild-type 0 |
| Stage of embryos | Morula |
Literature references
- Abnormal adherence junctions in the heart and reduced angiogenesis in transgenic mice overexpressing mutant type XIII collagen.;Sund M, Ylönen R, Tuomisto A, Sormunen R, Tahkola J, Kvist A P, Kontusaari S, Autio-Harmainen H, Pihlajaniemi T, ;2001;The EMBO journal;20;5153-64; 11566879
- Cardiac dysfunction in transgenic mouse fetuses overexpressing shortened type XIII collagen.;Tahkola Jenni, Räsänen Juha, Sund Malin, Mäkikallio Kaarin, Autio-Harmainen Helena, Pihlajaniemi Taina, ;2008;Cell and tissue research;333;61-9; 18481090
- A mutant collagen XIII alters intestinal expression of immune response genes and predisposes transgenic mice to develop B-cell lymphomas.;Tuomisto Anne, Sund Malin, Tahkola Jenni, Latvanlehto Anne, Savolainen Eeva-Riitta, Autio-Harmainen Helena, Liakka Annikki, Sormunen Raija, Vuoristo Jussi, West Anne, Lahesmaa Riitta, Morse Herbert C, Pihlajaniemi Taina, ;2008;Cancer research;68;10324-32; 19074901