B6NCrl;B6N-A^tm1Brd Cul4a^{tm1a(EUCOMM)Hmgu}/Ph

Status	Available to order
EMMA ID	EM:10587
Citation information	RRID:IMSR_EM:10587 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6NCrl;B6N-A^tm1Brd Cul4a^{tm1a(EUCOMM)Hmgu}/Ph
Alternative name	HEPD0649_1_F11
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Cul4a^{tm1a(EUCOMM)Hmgu}
Gene/Transgene symbol	Cul4a
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Institute of Molecular Genetics
Provider affiliation	Department of Transgenic Models of Diseases, Institute of Molecular Genetics
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0649_1_F11. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Institute of Molecular Genetics, Prague, Czech Republic

Disease and phenotype information

IMPC phenotypes (gene matching)

enhanced cued conditioning behavior / IMPC
small testis / IMPC
decreased spleen weight / IMPC
decreased exploration in new environment / IMPC
abnormal testis morphology / IMPC
abnormal retina morphology / IMPC
decreased mean platelet volume / IMPC
male infertility / IMPC
decreased circulating chloride level / IMPC
increased circulating potassium level / IMPC
increased fasting circulating glucose level / IMPC

MGI phenotypes (gene matching)

small testis / MGI
abnormal spermatogenesis / MGI
reduced female fertility / MGI
male infertility / MGI
decreased litter size / MGI
asthenozoospermia / MGI
oligozoospermia / MGI
abnormal cell cycle checkpoint function / MGI
decreased testis weight / MGI
small epididymis / MGI
azoospermia / MGI
abnormal male meiosis / MGI
reproductive system phenotype / MGI
abnormal spermatocyte morphology / MGI
abnormal spermatid morphology / MGI
abnormal DNA repair / MGI
abnormal male germ cell apoptosis / MGI
abnormal sperm flagellum morphology / MGI
abnormal sperm head morphology / MGI
abnormal double-strand DNA break repair / MGI
multinucleated giant male germ cells / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6NCrl;B6N-Atm1Brd Cul4atm1a(EUCOMM)Hmgu/Ph