C57BL/6N-Gdf15tm1c(KOMP)Wtsi/H

Status

Available to order

EMMA IDEM:10460
International strain nameC57BL/6N-Gdf15tm1c(KOMP)Wtsi/H
Alternative nameEPD0334_2_B10
Strain typeTargeted Mutant Strains : Targeted Conditional
Allele/Transgene symbolGdf15tm1c(KOMP)Wtsi
Gene/Transgene symbolGdf15
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider MRC, Medical Research Council
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis line originates from KOMP ES clone EPD0334_2_B10, after breeding with a Flp recombinase deleter line to convert the original targeted allele tm1a (knock-out first allele) into a conditional allele tm1c. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

MGI phenotypes (gene matching)
  • increased white adipose tissue amount / MGI
  • decreased motor neuron number / MGI
  • decreased sensory neuron number / MGI
  • abnormal L4 dorsal root ganglion morphology / MGI
  • abnormal L5 dorsal root ganglion morphology / MGI
  • abnormal trigeminal nerve morphology / MGI
  • abnormal facial nerve morphology / MGI
  • increased body weight / MGI
  • decreased body weight / MGI
  • hypoactivity / MGI
  • impaired coordination / MGI
  • increased urine glucose level / MGI
  • polyuria / MGI
  • abnormal kidney morphology / MGI
  • abnormal somatic nervous system morphology / MGI
  • increased myocardial infarction size / MGI
  • increased kidney weight / MGI
  • decreased lean body mass / MGI
  • decreased energy expenditure / MGI
  • abnormal thrombosis / MGI
  • homeostasis/metabolism phenotype / MGI
  • decreased bleeding time / MGI
  • decreased neuron number / MGI
  • abnormal basal metabolism / MGI
  • increased inguinal fat pad weight / MGI
  • increased mesenteric fat pad weight / MGI
  • increased retroperitoneal fat pad weight / MGI
  • increased platelet aggregation / MGI
  • increased sensitivity to induced morbidity/mortality / MGI
  • increased total body fat amount / MGI
  • increased food intake / MGI
  • increased fluid intake / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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