C57BL/6NTac-Nodaltm1a(EUCOMM)Wtsi/H

Status

Available to order

EMMA IDEM:10453
International strain nameC57BL/6NTac-Nodaltm1a(EUCOMM)Wtsi/H
Alternative nameEPD0197_2_B12
Strain typeTargeted Mutant Strains
Allele/Transgene symbolNodaltm1a(EUCOMM)Wtsi
Gene/Transgene symbolNodal
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider MRC, Medical Research Council
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0197_2_B12. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archivingheterozygous C57BL/6NTac
Breeding at archiving centreThis mutation was made on a coisogenic C57BL/6NTac background

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • corneal vascularization / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • increased circulating HDL cholesterol level / IMPC
  • embryonic lethality prior to tooth bud stage / IMPC
  • decreased locomotor activity / IMPC
  • increased grip strength / IMPC
  • increased circulating triglyceride level / IMPC
  • abnormal startle reflex / IMPC
  • increased large unstained cell number / IMPC
  • increased circulating cholesterol level / IMPC
MGI phenotypes (gene matching)
  • abnormal heart development / MGI
  • abnormal heart looping / MGI
  • abnormal interventricular septum morphology / MGI
  • enlarged pericardium / MGI
  • trabecula carnea hypoplasia / MGI
  • abnormal foregut morphology / MGI
  • right-sided isomerism / MGI
  • right pulmonary isomerism / MGI
  • abnormal liver morphology / MGI
  • small liver / MGI
  • pale liver / MGI
  • abnormal spleen morphology / MGI
  • small spleen / MGI
  • spleen hypoplasia / MGI
  • abnormal brain development / MGI
  • absent floor plate / MGI
  • absent notochord / MGI
  • decreased body size / MGI
  • abnormal ectoderm development / MGI
  • failure of primitive streak formation / MGI
  • abnormal gastrulation / MGI
  • decreased embryo size / MGI
  • abnormal embryo turning / MGI
  • abnormal left-right axis patterning / MGI
  • absent amniotic folds / MGI
  • embryonic growth arrest / MGI
  • pericardial edema / MGI
  • premature death / MGI
  • abnormal developmental patterning / MGI
  • abnormal embryonic tissue morphology / MGI
  • abnormal extraembryonic tissue morphology / MGI
  • no abnormal phenotype detected / MGI
  • abnormal gastrulation movements / MGI
  • abnormal primitive streak formation / MGI
  • abnormal primitive streak morphology / MGI
  • small ectoplacental cone / MGI
  • situs ambiguus / MGI
  • aortic hypertrophy / MGI
  • no phenotypic analysis / MGI
  • absent allantois / MGI
  • abnormal digestive system development / MGI
  • absent placental labyrinth / MGI
  • abnormal craniofacial development / MGI
  • thin myocardium compact layer / MGI
  • abnormal primitive node morphology / MGI
  • transposition of great arteries / MGI
  • heterotaxia / MGI
  • abnormal direction of heart looping / MGI
  • abnormal spongiotrophoblast layer morphology / MGI
  • abnormal embryonic neuroepithelium morphology / MGI
  • abnormal prechordal plate morphology / MGI
  • absent prechordal plate / MGI
  • truncated notochord / MGI
  • abnormal neural fold formation / MGI
  • absent amnion / MGI
  • holoprosencephaly / MGI
  • abnormal rostral-caudal axis patterning / MGI
  • embryo phenotype / MGI
  • right atrial isomerism / MGI
  • abnormal heart position or orientation / MGI
  • abnormal extraembryonic mesoderm development / MGI
  • absent somites / MGI
  • abnormal anterior visceral endoderm morphology / MGI
  • abnormal hindgut morphology / MGI
  • abnormal mesendoderm development / MGI
  • absent primitive node / MGI
  • increased trophoblast giant cell number / MGI
  • abnormal lateral plate mesoderm morphology / MGI
  • atrial septal defect / MGI
  • common atrium / MGI
  • abnormal extraembryonic ectoderm morphology / MGI
  • abnormal stomach position or orientation / MGI
  • neonatal lethality, complete penetrance / MGI
  • perinatal lethality, complete penetrance / MGI
  • prenatal lethality, complete penetrance / MGI
  • embryonic lethality between implantation and somite formation, complete penetrance / MGI
  • embryonic lethality between somite formation and embryo turning, complete penetrance / MGI
  • prenatal lethality, incomplete penetrance / MGI
  • embryonic lethality, incomplete penetrance / MGI
  • embryonic lethality during organogenesis, incomplete penetrance / MGI
  • abnormal visceral endoderm morphology / MGI
  • abnormal parietal yolk sac morphology / MGI
  • absent visceral yolk sac / MGI
  • thin placenta labyrinth / MGI
  • fused somites / MGI
  • absent heart tube / MGI
  • delayed heart development / MGI
  • absent foregut / MGI
  • truncated foregut / MGI
  • absent midbrain / MGI
  • increased spongiotrophoblast size / MGI
  • embryonic-extraembryonic boundary constriction / MGI
  • absent forebrain / MGI
  • rostral body truncation / MGI
  • abnormal head development / MGI
  • abnormal heart apex morphology / MGI
  • decreased embryonic neuroepithelial cell proliferation / MGI
  • absent head fold / MGI

Information on how we integrate external resources can be found here

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
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