C57BL/6N-Nras^{tm1a(EUCOMM)Hmgu}/H

Status	Available to order
EMMA ID	EM:10426
International strain name	C57BL/6N-Nras^{tm1a(EUCOMM)Hmgu}/H
Alternative name	HEPD0719_5_C04
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Nras^{tm1a(EUCOMM)Hmgu}
Gene/Transgene symbol	Nras
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0719_5_C04. For further details on the construction of this clone see the page at the IKMC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Large congenital melanocytic nevus / Orphanet_626
RAS-associated autoimmune leukoproliferative disease / Orphanet_268114
Juvenile myelomonocytic leukemia / Orphanet_86834
Differentiated thyroid carcinoma / Orphanet_146
Noonan syndrome / Orphanet_648
Langerhans cell histiocytosis / Orphanet_389

IMPC phenotypes (gene matching)

preweaning lethality, complete penetrance / IMPC
increased circulating glucose level / IMPC
increased circulating HDL cholesterol level / IMPC
short tibia / IMPC

MGI phenotypes (gene matching)

abnormal leukocyte cell number / MGI
decreased monocyte cell number / MGI
increased granulocyte number / MGI
pale liver / MGI
abnormal thymus morphology / MGI
decreased body weight / MGI
weight loss / MGI
postnatal growth retardation / MGI
no abnormal phenotype detected / MGI
no phenotypic analysis / MGI
decreased spleen weight / MGI
increased T cell number / MGI
abnormal granulocyte differentiation / MGI
preweaning lethality, complete penetrance / MGI
chylous ascites / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Nrastm1a(EUCOMM)Hmgu/H