B6;FVB-Tg(Hnrnpa2b1-rtTA2S*M2)9Jstr Tg(tetO-TNF)15Wxlr/Biat
| Status | Available to order |
| EMMA ID | EM:10368 |
| International strain name | B6;FVB-Tg(Hnrnpa2b1-rtTA2S*M2)9Jstr Tg(tetO-TNF)15Wxlr/Biat |
| Alternative name | ihTNFtg |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(tetO-TNF)15Wxlr, Tg(Hnrnpa2b1-rtTA2S*M2)9Jstr |
| Gene/Transgene symbol | Tg(tetO-TNF)15Wxlr, Tg(Hnrnpa2b1-rtTA2S*M2)9Jstr |
Information from provider
| Provider | Viktor Wixler |
| Provider affiliation | Institute of Molecular Virology, University Hospital Muenster |
| Additional owner | TET SYSTEMS HOLDING GMBH & CO KG having a place of business at Im Neuenheimer Feld 582, D-69120 Heidelberg, Germany and the the Erasmus MC Technology Transfer Office, 3000 CA Rotterdam, The Netherlands |
| Genetic information | Full-length human TNFalpha cDNA (Accession number NM_000594) was cloned behind the Tet-responsive P-tight promoter in the plasmid pTRE-Tight (BD Biosciences Clontech). The 3 prime UTR region of TNFalpha was replaced by the SV40 PolyA site, to avoid post-transcriptional regulation. The TNFalpha expression cassette under the Tet-inducible promoter was injected into the pronuclei of 0.5d FVB/N mouse oocytes. A positive Tg_ihTNFalpha founder was then crossed with transgenic mouse line hnRNPA2B1-rtTA2S-M2 (of C57BL/6 background) that was published earlier by Katsantoni et al. (2007). By further inbreeding, homozygous Tg_rtTA2/Tg_ihTNFalpha double transgenic mice were established and referred as ihTNFtg (see Retser et al.,2013 and Wixler et al., 2015). |
| Phenotypic information | Homozygous:The mice are fertile and do not display any abnormalities without doxycycline stimulation. On doxycycline administration with drinking water the inducible ihTNFtg mice developed an inflammatory arthritis- and psoriasis-like phenotype with fore and hind paws and the skin at head and neck area being prominently affected. The formation of “sausage”-like digits with obligatory involvement of the distal interphalangeal joints and nail malformation is very characteristic. Synovial hyperplasia, enthesitis, cartilage and bone alterations, formation of pannus tissue as well as inflammation of the skin epidermis and nail matrix appeared as early as one week after the treatment of mice with doxycycline and became aggravated over time. The abrogation of hTNFalpha by the removal of doxycycline after few weeks of stimulation resulted in a fast resolution of the most accomplished macroscopic and histological disorders.Heterozygous:as homozygous |
| Breeding history | The F1 animals were strictly intercrossed for more than 25 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
| Animals used for archiving | homozygous mixed, homozygous mixed |
| Breeding at archiving centre | Rederivation of shipped animals into SPF barrier and breeding of embryo donors |
| Stage of embryos | 2-cell |
Literature references
- Ubiquitous expression of the rtTA2S-M2 inducible system in transgenic mice driven by the human hnRNPA2B1/CBX3 CpG island.;Katsantoni Eleni Z, Anghelescu Nora E, Rottier Robbert, Moerland Matthijs, Antoniou Michael, de Crom Rini, Grosveld Frank, Strouboulis John, ;2007;BMC developmental biology;7;108; 17900353
- Doxycycline-induced expression of transgenic human tumor necrosis factor α in adult mice results in psoriasis-like arthritis.;Retser Eugen, Schied Tanja, Skryabin Boris V, Vogl Thomas, Kanczler Janos M, Hamann Nina, Niehoff Anja, Hermann Sven, Eisenblätter Michel, Wachsmuth Lydia, Pap Thomas, van Lent Peter L E M, Loser Karin, Roth Johannes, Zaucke Frank, Ludwig Stephan, Wixler Viktor, ;2013;Arthritis and rheumatism;65;2290-300; 23740547
- FHL2 regulates the resolution of tissue damage in chronic inflammatory arthritis.;Wixler Viktor, Cromme Christoph, Retser Eugen, Meyer Lars-Henrik, Smyth Neil, Mühlenberg Katja, Korb-Pap Adelheid, Koers-Wunrau Christina, Sotsios Yannis, Bassel-Duby Rhonda, Baeten Dominique, Tak Paul P, Niederreiter Birgit, Redlich Kurt, Bertrand Jessica, Skryabin Boris V, Ludwig Stephan, Pap Thomas, ;2015;Annals of the rheumatic diseases;74;2216-23; 25125695
- Macrophage-mediated psoriasis can be suppressed by regulatory T lymphocytes.;Leite Dantas Rafael, Masemann Dörthe, Schied Tanja, Bergmeier Vera, Vogl Thomas, Loser Karin, Brachvogel Bent, Varga Georg, Ludwig Stephan, Wixler Viktor, ;2016;The Journal of pathology;240;366-377; 27555499