C57BL/6N-Acantm1a(EUCOMM)Hmgu/H

Status

Available to order

EMMA IDEM:10224
International strain nameC57BL/6N-Acantm1a(EUCOMM)Hmgu/H
Alternative nameHEPD0602_5_G11
Strain typeTargeted Mutant Strains
Allele/Transgene symbolAcantm1a(EUCOMM)Hmgu
Gene/Transgene symbolAcan
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider MRC, Medical Research Council
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis mouse line originates from EUCOMM ES clone HEPD0602_5_G11. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
References
  • Aggrecan Directs Extracellular Matrix-Mediated Neuronal Plasticity.;Rowlands Daire, Lensjø Kristian K, Dinh Tovy, Yang Sujeong, Andrews Melissa R, Hafting Torkel, Fyhn Marianne, Fawcett James W, Dick Gunnar, ;2018;The Journal of neuroscience : the official journal of the Society for Neuroscience;38;10102-10113; 30282728

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archivingheterozygous C57BL/6NTac
Breeding at archiving centreThe mutation is coisogenic on a C57BL/6NTac background

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • increased circulating HDL cholesterol level / IMPC
  • preweaning lethality, complete penetrance / IMPC
MGI phenotypes (gene matching)
  • abnormal organ of Corti morphology / MGI
  • cleft palate / MGI
  • abnormal trabecular bone morphology / MGI
  • abnormal vertebrae morphology / MGI
  • abnormal vertebral body morphology / MGI
  • abnormal rib morphology / MGI
  • kyphosis / MGI
  • lordosis / MGI
  • abnormal cartilage development / MGI
  • abnormal chondrocyte morphology / MGI
  • short snout / MGI
  • flattened snout / MGI
  • short limbs / MGI
  • abnormal liver morphology / MGI
  • enlarged liver / MGI
  • abnormal spinal cord morphology / MGI
  • abnormal lung morphology / MGI
  • pulmonary hypoplasia / MGI
  • obese / MGI
  • distended abdomen / MGI
  • abnormal gait / MGI
  • aphagia / MGI
  • cardiac hypertrophy / MGI
  • respiratory failure / MGI
  • respiratory distress / MGI
  • deafness / MGI
  • premature death / MGI
  • abnormal limb morphology / MGI
  • abnormal craniofacial bone morphology / MGI
  • no abnormal phenotype detected / MGI
  • disproportionate dwarf / MGI
  • brachydactyly / MGI
  • brachypodia / MGI
  • abnormal joint morphology / MGI
  • short femur / MGI
  • abnormal tracheal cartilage morphology / MGI
  • decreased susceptibility to induced arthritis / MGI
  • polyhydramnios / MGI
  • abnormal intervertebral disk morphology / MGI
  • short humerus / MGI
  • abnormal cochlear inner hair cell morphology / MGI
  • absent cochlear outer hair cells / MGI
  • absent caudal vertebrae / MGI
  • intervertebral disk degeneration / MGI
  • decreased length of long bones / MGI
  • short vertebral column / MGI
  • small thoracic cavity / MGI
  • abnormal cochlear IHC afferent innervation pattern / MGI
  • cardiomyopathy / MGI
  • abnormal limb development / MGI
  • protruding tongue / MGI
  • neonatal lethality, complete penetrance / MGI
  • increased or absent threshold for auditory brainstem response / MGI
  • short basicranium / MGI

Literature references

  • Aggrecan Directs Extracellular Matrix-Mediated Neuronal Plasticity.;Rowlands Daire, Lensjø Kristian K, Dinh Tovy, Yang Sujeong, Andrews Melissa R, Hafting Torkel, Fyhn Marianne, Fawcett James W, Dick Gunnar, ;2018;The Journal of neuroscience : the official journal of the Society for Neuroscience;38;10102-10113; 30282728
  • Astrocytes require perineuronal nets to maintain synaptic homeostasis in mice.;Tewari Bhanu P, Woo AnnaLin M, Prim Courtney E, Chaunsali Lata, Patel Dipan C, Kimbrough Ian F, Engel Kaliroi, Browning Jack L, Campbell Susan L, Sontheimer Harald, ;2024;Nature neuroscience;27;1475-1488; 39020018

Information on how we integrate external resources can be found here

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

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