C57BL/6N-A^tm1Brd Fads3^{tm1a(EUCOMM)Hmgu}/WtsiCnrm

Status	Available to order
EMMA ID	EM:10193
International strain name	C57BL/6N-A^tm1Brd Fads3^{tm1a(EUCOMM)Hmgu}/WtsiCnrm
Alternative name	HEPD0959_5_A07
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Fads3^{tm1a(EUCOMM)Hmgu}
Gene/Transgene symbol	Fads3
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0959_5_A07. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

IMPC phenotypes (gene matching)

abnormal auditory brainstem response / IMPC
decreased circulating phosphate level / IMPC
absent pinna reflex / IMPC

MGI phenotypes (gene matching)

increased fatty acid level / MGI
decreased fatty acid level / MGI
homeostasis/metabolism phenotype / MGI
growth/size/body region phenotype / MGI
behavior/neurological phenotype / MGI
reproductive system phenotype / MGI
increased liver free fatty acids level / MGI
decreased liver free fatty acids level / MGI
mortality/aging / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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INFRAFRONTIER^® and European Mouse Mutant Archive - EMMA^® are registered trademarks at the European Union Intellectual Property Office (EUIPO).

C57BL/6N-Atm1Brd Fads3tm1a(EUCOMM)Hmgu/WtsiCnrm