C57BL/6NTac-Pdzk1tm2a(EUCOMM)Wtsi/WtsiCnrm

Status

Available to order

EMMA IDEM:10187
Citation informationRRID:IMSR_EM:10187 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6NTac-Pdzk1tm2a(EUCOMM)Wtsi/WtsiCnrm
Alternative nameBEPD0005_1_D09
Strain typeTargeted Mutant Strains
Allele/Transgene symbolPdzk1tm2a(EUCOMM)Wtsi
Gene/Transgene symbolPdzk1
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone BEPD0005_1_D09. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

IMPC phenotypes (gene matching)
  • increased circulating LDL cholesterol level / IMPC
  • increased circulating HDL cholesterol level / IMPC
  • preweaning lethality, incomplete penetrance / IMPC
  • increased circulating cholesterol level / IMPC
MGI phenotypes (gene matching)
  • polydactyly / MGI
  • abnormal liver morphology / MGI
  • abnormal forebrain morphology / MGI
  • abnormal lymphatic vessel morphology / MGI
  • abnormal eye muscle morphology / MGI
  • abnormal Mullerian duct morphology / MGI
  • abnormal Wolffian duct morphology / MGI
  • fusion of vertebral arches / MGI
  • increased circulating cholesterol level / MGI
  • abnormal circulating protein level / MGI
  • ostium primum atrial septal defect / MGI
  • atrioventricular septal defect / MGI
  • anomalous pulmonary venous connection / MGI
  • absent segment of posterior cerebral artery / MGI
  • subcutaneous edema / MGI
  • abnormal vitelline vein connection / MGI
  • abnormal infrahyoid muscle connection / MGI
  • absent connection between subcutaneous lymph vessels and lymph sac / MGI
  • blood in lymph vessels / MGI
  • symmetric azygos veins / MGI
  • double lumen aortic arch / MGI
  • abnormal vertebral artery topology / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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