IMPC phenotypes (gene matching)
B6.Cg-Rasgrf1tm1Kln/Cnrm
| Status | Available to order |
| EMMA ID | EM:10171 |
| Citation information | RRID:IMSR_EM:10171 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Rasgrf1tm1Kln/Cnrm |
| Alternative name | Rasgrf1 KO |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Rasgrf1tm1Kln |
| Gene/Transgene symbol | Rasgrf1 |
Information from provider
| Provider | Riccardo Brambilla |
| Provider affiliation | School of Biosciences, Cardiff University |
| Genetic information | Genetic inactivation via Homologous Recombination in ES cells of the Rasgrf1 locus. Exon 3-5 were replaced by a Neomycin Cassette. |
| Phenotypic information | Homozygous:Ras-GRF1 KO mice showed learning and deficit phenotypes, and alterations of the responses to drugs of abuse. Moreover they showed altered response to L-DOPA induced dyskinesia. Mutant mice are also smaller (10% reduction of body weight) than the littermate control.Heterozygous:The RasGRF1 locus is genetically imprinted (paternal allele-specific expression). Therefore, depending on the breeding protocol, some heterozygous mice are functionally KO. |
| Breeding history | Animals were generated at the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany, in 1995 and then transferred in 1998 to the San Raffaele Scientific Institute in Milan, Italy |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
MGI phenotypes (allele matching)
- reduced long term potentiation / MGI
- impaired synaptic plasticity / MGI
- abnormal active avoidance behavior / MGI
- abnormal excitatory postsynaptic potential / MGI
- nervous system phenotype / MGI
- behavior/neurological phenotype / MGI
- abnormal long term spatial reference memory / MGI
- impaired contextual conditioning behavior / MGI
MGI phenotypes (gene matching)
- decreased body weight / MGI
- abnormal spatial learning / MGI
- reduced long term potentiation / MGI
- postnatal growth retardation / MGI
- abnormal pituitary secretion / MGI
- impaired synaptic plasticity / MGI
- no abnormal phenotype detected / MGI
- decreased circulating insulin level / MGI
- abnormal active avoidance behavior / MGI
- abnormal discrimination learning / MGI
- abnormal excitatory postsynaptic potential / MGI
- impaired social transmission of food preference / MGI
- no phenotypic analysis / MGI
- maternal imprinting / MGI
- paternal imprinting / MGI
- decreased pancreatic beta cell number / MGI
- nervous system phenotype / MGI
- abnormal imprinting / MGI
- decreased circulating insulin-like growth factor I level / MGI
- decreased growth hormone level / MGI
- impaired glucose tolerance / MGI
- behavior/neurological phenotype / MGI
- enhanced lipolysis / MGI
- abnormal long term spatial reference memory / MGI
- abnormal DNA methylation / MGI
- small pancreatic islets / MGI
- impaired contextual conditioning behavior / MGI
- decreased total body fat amount / MGI
Literature references
- A role for the Ras signalling pathway in synaptic transmission and long-term memory.;Brambilla R, Gnesutta N, Minichiello L, White G, Roylance A J, Herron C E, Ramsey M, Wolfer D P, Cestari V, Rossi-Arnaud C, Grant S G, Chapman P F, Lipp H P, Sturani E, Klein R, ;1997;Nature;390;281-6; 9384379
- Ras-guanine nucleotide-releasing factor 1 (Ras-GRF1) controls activation of extracellular signal-regulated kinase (ERK) signaling in the striatum and long-term behavioral responses to cocaine.;Fasano Stefania, D'Antoni Angela, Orban Paul C, Valjent Emmanuel, Putignano Elena, Vara Hugo, Pizzorusso Tommaso, Giustetto Maurizio, Yoon Bongjune, Soloway Paul, Maldonado Rafael, Caboche Jocelyne, Brambilla Riccardo, ;2009;Biological psychiatry;66;758-68; 19446794
- Inhibition of Ras-guanine nucleotide-releasing factor 1 (Ras-GRF1) signaling in the striatum reverts motor symptoms associated with L-dopa-induced dyskinesia.;Fasano Stefania, Bezard Erwan, D'Antoni Angela, Francardo Veronica, Indrigo Marzia, Qin Li, Doveró Sandra, Cerovic Milica, Cenci M Angela, Brambilla Riccardo, ;2010;Proceedings of the National Academy of Sciences of the United States of America;107;21824-9; 21115823
- Mice Lacking Ras-GRF1 Show Contextual Fear Conditioning but not Spatial Memory Impairments: Convergent Evidence from Two Independently Generated Mouse Mutant Lines.;d'Isa Raffaele, Clapcote Steven J, Voikar Vootele, Wolfer David P, Giese Karl Peter, Brambilla Riccardo, Fasano Stefania, ;2011;Frontiers in behavioral neuroscience;5;78; 22164138
- Derangement of Ras-guanine nucleotide-releasing factor 1 (Ras-GRF1) and extracellular signal-regulated kinase (ERK) dependent striatal plasticity in L-DOPA-induced dyskinesia.;Cerovic Milica, Bagetta Vincenza, Pendolino Valentina, Ghiglieri Veronica, Fasano Stefania, Morella Ilaria, Hardingham Neil, Heuer Andreas, Papale Alessandro, Marchisella Francesca, Giampà Carmela, Calabresi Paolo, Picconi Barbara, Brambilla Riccardo, ;2015;Biological psychiatry;77;106-15; 24844602
- Differential involvement of Ras-GRF1 and Ras-GRF2 in L-DOPA-induced dyskinesia.;Bido Simone, Solari Nicola, Indrigo Marzia, D'Antoni Angela, Brambilla Riccardo, Morari Michele, Fasano Stefania, ;2015;Annals of clinical and translational neurology;2;662-78; 26125041