B6.Cg-Rasgrf1tm1Kln/Cnrm
Status | Available to order |
EMMA ID | EM:10171 |
International strain name | B6.Cg-Rasgrf1tm1Kln/Cnrm |
Alternative name | Rasgrf1 KO |
Strain type | Targeted Mutant Strains : Knock-out |
Allele/Transgene symbol | Rasgrf1tm1Kln |
Gene/Transgene symbol | Rasgrf1 |
Information from provider
Provider | Riccardo Brambilla |
Provider affiliation | School of Biosciences, Cardiff University |
Genetic information | Genetic inactivation via Homologous Recombination in ES cells of the Rasgrf1 locus. Exon 3-5 were replaced by a Neomycin Cassette. |
Phenotypic information | Homozygous:Ras-GRF1 KO mice showed learning and deficit phenotypes, and alterations of the responses to drugs of abuse. Moreover they showed altered response to L-DOPA induced dyskinesia. Mutant mice are also smaller (10% reduction of body weight) than the littermate control.Heterozygous:The RasGRF1 locus is genetically imprinted (paternal allele-specific expression). Therefore, depending on the breeding protocol, some heterozygous mice are functionally KO. |
Breeding history | Animals were generated at the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany, in 1995 and then transferred in 1998 to the San Raffaele Scientific Institute in Milan, Italy |
References |
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Homozygous fertile | yes |
Homozygous viable | yes |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
IMPC phenotypes (gene matching)
MGI phenotypes (allele matching)
- reduced long term potentiation / MGI
- impaired synaptic plasticity / MGI
- abnormal active avoidance behavior / MGI
- abnormal excitatory postsynaptic potential / MGI
- nervous system phenotype / MGI
- behavior/neurological phenotype / MGI
- abnormal long term spatial reference memory / MGI
- impaired contextual conditioning behavior / MGI
MGI phenotypes (gene matching)
- decreased body weight / MGI
- abnormal spatial learning / MGI
- reduced long term potentiation / MGI
- postnatal growth retardation / MGI
- abnormal pituitary secretion / MGI
- impaired synaptic plasticity / MGI
- no abnormal phenotype detected / MGI
- decreased circulating insulin level / MGI
- abnormal active avoidance behavior / MGI
- abnormal discrimination learning / MGI
- abnormal excitatory postsynaptic potential / MGI
- impaired social transmission of food preference / MGI
- no phenotypic analysis / MGI
- maternal imprinting / MGI
- paternal imprinting / MGI
- decreased pancreatic beta cell number / MGI
- nervous system phenotype / MGI
- abnormal imprinting / MGI
- decreased circulating insulin-like growth factor I level / MGI
- decreased growth hormone level / MGI
- impaired glucose tolerance / MGI
- behavior/neurological phenotype / MGI
- enhanced lipolysis / MGI
- abnormal long term spatial reference memory / MGI
- abnormal DNA methylation / MGI
- small pancreatic islets / MGI
- impaired contextual conditioning behavior / MGI
- decreased total body fat amount / MGI
Literature references
- A role for the Ras signalling pathway in synaptic transmission and long-term memory.;Brambilla R, Gnesutta N, Minichiello L, White G, Roylance A J, Herron C E, Ramsey M, Wolfer D P, Cestari V, Rossi-Arnaud C, Grant S G, Chapman P F, Lipp H P, Sturani E, Klein R, ;1997;Nature;390;281-6; 9384379
- Ras-guanine nucleotide-releasing factor 1 (Ras-GRF1) controls activation of extracellular signal-regulated kinase (ERK) signaling in the striatum and long-term behavioral responses to cocaine.;Fasano Stefania, D'Antoni Angela, Orban Paul C, Valjent Emmanuel, Putignano Elena, Vara Hugo, Pizzorusso Tommaso, Giustetto Maurizio, Yoon Bongjune, Soloway Paul, Maldonado Rafael, Caboche Jocelyne, Brambilla Riccardo, ;2009;Biological psychiatry;66;758-68; 19446794
- Inhibition of Ras-guanine nucleotide-releasing factor 1 (Ras-GRF1) signaling in the striatum reverts motor symptoms associated with L-dopa-induced dyskinesia.;Fasano Stefania, Bezard Erwan, D'Antoni Angela, Francardo Veronica, Indrigo Marzia, Qin Li, Doveró Sandra, Cerovic Milica, Cenci M Angela, Brambilla Riccardo, ;2010;Proceedings of the National Academy of Sciences of the United States of America;107;21824-9; 21115823
- Mice Lacking Ras-GRF1 Show Contextual Fear Conditioning but not Spatial Memory Impairments: Convergent Evidence from Two Independently Generated Mouse Mutant Lines.;d'Isa Raffaele, Clapcote Steven J, Voikar Vootele, Wolfer David P, Giese Karl Peter, Brambilla Riccardo, Fasano Stefania, ;2011;Frontiers in behavioral neuroscience;5;78; 22164138
- Derangement of Ras-guanine nucleotide-releasing factor 1 (Ras-GRF1) and extracellular signal-regulated kinase (ERK) dependent striatal plasticity in L-DOPA-induced dyskinesia.;Cerovic Milica, Bagetta Vincenza, Pendolino Valentina, Ghiglieri Veronica, Fasano Stefania, Morella Ilaria, Hardingham Neil, Heuer Andreas, Papale Alessandro, Marchisella Francesca, Giampà Carmela, Calabresi Paolo, Picconi Barbara, Brambilla Riccardo, ;2015;Biological psychiatry;77;106-15; 24844602
- Differential involvement of Ras-GRF1 and Ras-GRF2 in L-DOPA-induced dyskinesia.;Bido Simone, Solari Nicola, Indrigo Marzia, D'Antoni Angela, Brambilla Riccardo, Morari Michele, Fasano Stefania, ;2015;Annals of clinical and translational neurology;2;662-78; 26125041
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