B6J;129-Kcns1tm1.1Kcl Tg(Avil-icre/ERT2)AJwo/H
| Status | Available to order |
| EMMA ID | EM:10168 |
| International strain name | B6J;129-Kcns1tm1.1Kcl Tg(Avil-icre/ERT2)AJwo/H |
| Alternative name | AdvCre-Kcns1 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Avil-icre/ERT2)AJwo, Kcns1tm1.1Kcl |
| Gene/Transgene symbol | Tg(Avil-icre/ERT2)AJwo, Kcns1 |
Information from provider
| Provider | Stephen Mcmahon |
| Provider affiliation | Neurorestoration Group, King |
| Genetic information | This mouse strain is homozygous for floxed Kcns1 and hemizygous for AdvCreERT2 BAC transgene. The BAC transgene was introduced to a 129 mouse line and the resulting line was then backcrossed to C57BL/6J. The targeting of Kcns1 was performed in 129S7/SvEv ES cells. The resulting line was then crossed to a flp recombinase-expressing line on a C57BL/6J background (code H109) to remove the neo cassette. The stocks were then intercrossed (and backcrossed) to produce the AdvCre-Kcns1 line (on a mixed 129 & C57BL/6J background). |
| Phenotypic information | Homozygous:noneHeterozygous:none |
| Breeding history | The targeting of Kcns1 was performed in 129S7/SvEv ES cells. The resulting line was then crossed to a flp recombinase-expressing line on a C57BL/6J background (code H109) to remove the neo cassette. The stocks were then intercrossed (and backcrossed) to produce the AdvCre-Kcns1 line (on a mixed 129 & C57BL/6J background). |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
| Animals used for archiving | heterozygous C57BL/6J |
| Breeding at archiving centre | Intercrossed |