C57BL/6N-Prdm4tm1c(EUCOMM)Hmgu/H

Status

Available to order

EMMA IDEM:10148
Citation informationRRID:IMSR_EM:10148 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6N-Prdm4tm1c(EUCOMM)Hmgu/H
Alternative nameHEPD0557_7_B10
Strain typeTargeted Mutant Strains : Targeted Conditional
Allele/Transgene symbolPrdm4tm1c(EUCOMM)Hmgu
Gene/Transgene symbolPrdm4
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider MRC, Medical Research Council
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis line originates from EUCOMM ES clone HEPD0557_7_B10, after breeding with a Flp recombinase deleter line to convert the original targeted allele tm1a (knock-out first allele) into a conditional allele tm1c. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)
  • short tibia / IMPC
  • decreased total body fat amount / IMPC
  • increased cardiac muscle contractility / IMPC
  • abnormal cornea morphology / IMPC
  • microphthalmia / IMPC
  • impaired glucose tolerance / IMPC
  • abnormal heart left ventricle morphology / IMPC
  • enlarged urinary bladder / IMPC
  • absent spleen / IMPC
  • abnormal iris morphology / IMPC
  • increased lean body mass / IMPC
  • abnormal gait / IMPC
  • increased circulating amylase level / IMPC
  • thick ventricular wall / IMPC
MGI phenotypes (gene matching)
  • no abnormal phenotype detected / MGI
  • no phenotypic analysis / MGI

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Availabilities

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Practical information

Genotyping protocol

Example health report
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