C57BL/6N-Uqcrh^{tm1b(EUCOMM)Wtsi}/Ieg

Status	Available to order
EMMA ID	EM:10141
International strain name	C57BL/6N-Uqcrh^{tm1b(EUCOMM)Wtsi}/Ieg
Alternative name	EPD0378_3_C07
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Uqcrh^{tm1b(EUCOMM)Wtsi}
Gene/Transgene symbol	Uqcrh
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from EUCOMM ES clone EPD0378_3_C07. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele (Gt(ROSA)26Sortm1(ACTB-cre,-EGFP)Ics (MGI:5285392)). Click here for more information on EUCOMM final vectors.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Characterising a homozygous two-exon deletion in UQCRH: comparing human and mouse phenotypes.;Vidali Silvia, Gerlini Raffaele, Thompson Kyle, Urquhart Jill E, Meisterknecht Jana, Aguilar-Pimentel Juan Antonio, Amarie Oana V, Becker Lore, Breen Catherine, Calzada-Wack Julia, Chhabra Nirav F, Cho Yi-Li, da Silva-Buttkus Patricia, Feichtinger René G, Gampe Kristine, Garrett Lillian, Hoefig Kai P, Hölter Sabine M, Jameson Elisabeth, Klein-Rodewald Tanja, Leuchtenberger Stefanie, Marschall Susan, Mayer-Kuckuk Philipp, Miller Gregor, Oestereicher Manuela A, Pfannes Kristina, Rathkolb Birgit, Rozman Jan, Sanders Charlotte, Spielmann Nadine, Stoeger Claudia, Szibor Marten, Treise Irina, Walter John H, Wurst Wolfgang, Mayr Johannes A, Fuchs Helmut, Gärtner Ulrich, Wittig Ilka, Taylor Robert W, Newman William G, Prokisch Holger, Gailus-Durner Valerie, Hrabě de Angelis Martin, ;2021;EMBO molecular medicine;13;e14397; 34750991 Knockout of the Complex III subunit Uqcrh causes bioenergetic impairment and cardiac contractile dysfunction.;Spielmann Nadine, Schenkl Christina, Komlódi Tímea, da Silva-Buttkus Patricia, Heyne Estelle, Rohde Jana, Amarie Oana V, Rathkolb Birgit, Gnaiger Erich, Doenst Torsten, Fuchs Helmut, Gailus-Durner Valérie, de Angelis Martin Hrabě, Szibor Marten, ;2023;Mammalian genome : official journal of the International Mammalian Genome Society;34;229-243; 36565314

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Literature references

Characterising a homozygous two-exon deletion in UQCRH: comparing human and mouse phenotypes.;Vidali Silvia, Gerlini Raffaele, Thompson Kyle, Urquhart Jill E, Meisterknecht Jana, Aguilar-Pimentel Juan Antonio, Amarie Oana V, Becker Lore, Breen Catherine, Calzada-Wack Julia, Chhabra Nirav F, Cho Yi-Li, da Silva-Buttkus Patricia, Feichtinger René G, Gampe Kristine, Garrett Lillian, Hoefig Kai P, Hölter Sabine M, Jameson Elisabeth, Klein-Rodewald Tanja, Leuchtenberger Stefanie, Marschall Susan, Mayer-Kuckuk Philipp, Miller Gregor, Oestereicher Manuela A, Pfannes Kristina, Rathkolb Birgit, Rozman Jan, Sanders Charlotte, Spielmann Nadine, Stoeger Claudia, Szibor Marten, Treise Irina, Walter John H, Wurst Wolfgang, Mayr Johannes A, Fuchs Helmut, Gärtner Ulrich, Wittig Ilka, Taylor Robert W, Newman William G, Prokisch Holger, Gailus-Durner Valerie, Hrabě de Angelis Martin, ;2021;EMBO molecular medicine;13;e14397; 34750991
Knockout of the Complex III subunit Uqcrh causes bioenergetic impairment and cardiac contractile dysfunction.;Spielmann Nadine, Schenkl Christina, Komlódi Tímea, da Silva-Buttkus Patricia, Heyne Estelle, Rohde Jana, Amarie Oana V, Rathkolb Birgit, Gnaiger Erich, Doenst Torsten, Fuchs Helmut, Gailus-Durner Valérie, de Angelis Martin Hrabě, Szibor Marten, ;2023;Mammalian genome : official journal of the International Mammalian Genome Society;34;229-243; 36565314

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Uqcrhtm1b(EUCOMM)Wtsi/Ieg