C57BL/6N-Pax5tm1b(EUCOMM)Wtsi/Ieg

Status

Available to order

EMMA IDEM:10134
International strain nameC57BL/6N-Pax5tm1b(EUCOMM)Wtsi/Ieg
Alternative nameEPD0237_3_F11
Strain typeTargeted Mutant Strains
Allele/Transgene symbolPax5tm1b(EUCOMM)Wtsi
Gene/Transgene symbolPax5
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliationInstitute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0237_3_F11. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele (Gt(ROSA)26Sortm1(ACTB-cre,-EGFP)Ics (MGI:5285392)). Click here for more information on EUCOMM final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivingheterozygous C57BL/6NTac

Disease and phenotype information

IMPC phenotypes (allele matching)
  • decreased leukocyte cell number / IMPC
  • hyperactivity / IMPC
  • increased circulating triglyceride level / IMPC
  • abnormal auditory brainstem response / IMPC
  • impaired glucose tolerance / IMPC
  • immune system phenotype / IMPC
  • increased circulating amylase level / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • increased circulating lipase level / IMPC
  • increased CD4-positive NK T cell number / IMPC
  • increased memory-marker CD4-positive NK T cell number / IMPC
IMPC phenotypes (gene matching)
  • increased circulating lipase level / IMPC
  • increased CD4-positive NK T cell number / IMPC
  • increased memory-marker CD4-positive NK T cell number / IMPC
  • impaired glucose tolerance / IMPC
  • abnormal auditory brainstem response / IMPC
  • increased circulating amylase level / IMPC
  • increased circulating triglyceride level / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • decreased leukocyte cell number / IMPC
  • hyperactivity / IMPC
  • immune system phenotype / IMPC
MGI phenotypes (gene matching)
  • increased monocyte cell number / MGI
  • abnormal cerebellum morphology / MGI
  • abnormal cerebellar foliation / MGI
  • abnormal cerebellum vermis morphology / MGI
  • decreased body weight / MGI
  • decreased body size / MGI
  • limb grasping / MGI
  • postnatal growth retardation / MGI
  • abnormal humoral immune response / MGI
  • arrested B cell differentiation / MGI
  • decreased IgG level / MGI
  • decreased IgM level / MGI
  • decreased IgA level / MGI
  • premature death / MGI
  • abnormal B cell differentiation / MGI
  • no abnormal phenotype detected / MGI
  • abnormal lymphopoiesis / MGI
  • abnormal B cell physiology / MGI
  • decreased immunoglobulin level / MGI
  • decreased IgD level / MGI
  • abnormal cell migration / MGI
  • abnormal cell adhesion / MGI
  • increased B-1 B cell number / MGI
  • decreased B-1 B cell number / MGI
  • increased T cell number / MGI
  • decreased B cell number / MGI
  • abnormal late pro-B cell / MGI
  • hearing/vestibular/ear phenotype / MGI
  • immune system phenotype / MGI
  • increased macrophage cell number / MGI
  • absent late pro-B cells / MGI
  • abnormal cerebellar lobule formation / MGI
  • abnormal hindbrain development / MGI
  • increased single-positive T cell number / MGI
  • decreased B-1b cell number / MGI
  • absent B-2 B cells / MGI
  • absent mature B cells / MGI
  • decreased immature B cell number / MGI
  • decreased IgG1 level / MGI
  • decreased IgG2a level / MGI
  • decreased IgG2b level / MGI
  • abnormal immunoglobulin heavy chain V(D)J recombination / MGI
  • decreased inferior colliculus size / MGI
  • increased CD4-positive, CD25-positive, alpha-beta regulatory T cell number / MGI
  • increased CD8-positive, alpha-beta memory T cell number / MGI
  • lethality at weaning, complete penetrance / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • impaired humoral immune response / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.


Search
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).