C57BL/6N-Cyp7a1^{tm1b(EUCOMM)Wtsi}/Ieg

Status	Available to order
EMMA ID	EM:10117
International strain name	C57BL/6N-Cyp7a1^{tm1b(EUCOMM)Wtsi}/Ieg
Alternative name	EPD0426_3_C04
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Cyp7a1^{tm1b(EUCOMM)Wtsi}
Gene/Transgene symbol	Cyp7a1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from EUCOMM ES clone EPD0426_3_C04. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele (Gt(ROSA)26Sor). Click here for more information on EUCOMM final vectors.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6NTac

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Hypercholesterolemia due to cholesterol 7alpha-hydroxylase deficiency / Orphanet_209902

IMPC phenotypes (allele matching)

increased leukocyte cell number / IMPC
persistence of hyaloid vascular system / IMPC
abnormal lens morphology / IMPC
cataract / IMPC
abnormal retina morphology / IMPC
decreased exploration in new environment / IMPC
increased startle reflex / IMPC
increased circulating HDL cholesterol level / IMPC
abnormal vitreous body morphology / IMPC
abnormal retina vasculature morphology / IMPC
increased circulating alkaline phosphatase level / IMPC
decreased circulating iron level / IMPC
increased circulating cholesterol level / IMPC
increased circulating bilirubin level / IMPC
decreased circulating serum albumin level / IMPC
decreased blood urea nitrogen level / IMPC
abnormal retina blood vessel morphology / IMPC
increased circulating unsaturated transferrin level / IMPC

IMPC phenotypes (gene matching)

increased circulating HDL cholesterol level / IMPC
abnormal retina blood vessel morphology / IMPC
increased startle reflex / IMPC
abnormal retina vasculature morphology / IMPC
decreased circulating serum albumin level / IMPC
increased circulating alkaline phosphatase level / IMPC
abnormal retina morphology / IMPC
persistence of hyaloid vascular system / IMPC
increased circulating bilirubin level / IMPC
abnormal lens morphology / IMPC
decreased circulating iron level / IMPC
abnormal vitreous body morphology / IMPC
decreased blood urea nitrogen level / IMPC
cataract / IMPC
increased leukocyte cell number / IMPC
decreased exploration in new environment / IMPC
increased circulating cholesterol level / IMPC
increased circulating unsaturated transferrin level / IMPC

MGI phenotypes (gene matching)

abnormal liver morphology / MGI
abnormal liver physiology / MGI
abnormal skin condition / MGI
flaky skin / MGI
oily skin / MGI
abnormal epidermis stratum basale morphology / MGI
thin epidermis stratum spinosum / MGI
abnormal epidermis stratum granulosum morphology / MGI
hyperkeratosis / MGI
thin dermal layer / MGI
delayed eyelid opening / MGI
hypoactivity / MGI
decreased exploration in new environment / MGI
postnatal growth retardation / MGI
abnormal skin morphology / MGI
abnormal vision / MGI
abnormal lipid homeostasis / MGI
decreased intestinal cholesterol absorption / MGI
steatorrhea / MGI
greasy coat / MGI
dry skin / MGI
abnormal bile composition / MGI
abnormal bile salt level / MGI
abnormal bile salt homeostasis / MGI
abnormal fat-soluble vitamin level / MGI
decreased birth weight / MGI
postnatal lethality, incomplete penetrance / MGI
perinatal lethality, incomplete penetrance / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

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C57BL/6N-Cyp7a1tm1b(EUCOMM)Wtsi/Ieg