C57BL/6NTac-Nphs2tm2(EGFP/cre/ERT2)Wtsi/WtsiIeg

Status

Available to order

EMMA IDEM:10065
International strain nameC57BL/6NTac-Nphs2tm2(EGFP/cre/ERT2)Wtsi/WtsiIeg
Alternative nameCEPD0091_1_C09
Strain type
Allele/Transgene symbolNphs2tm2(EGFP/cre/ERT2)Wtsi
Gene/Transgene symbolNphs2

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone CEPD0091_1_C09. For further details on the construction of this clone see the page at the IMPC portal.
Quality Control/Disclaimer: The structure of the targeted mutation is not fully verified. It is recommended that the recipient confirms the mutation structure by Southern blotting. The Cre expression pattern is expected to mirror that of the host gene, but this may not be fully described and/or may be modified in the targeted allele. Although characterisation of the Cre expression pattern is an objective of EUCOMMTools this is an ongoing process which may be incomplete. It is therefore recommended that the recipient verifies the Cre expression pattern. For the tm2(EGFP/Cre/ERT2) type allele, if the promoter-driven selection cassette is flanked with Rox sites, it may be removed using Dre recombinase. Please be aware that the strain description may be subject to change. For the most recent description please consult the information pages for the appropriate product details at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (gene matching)
  • abnormal kidney cortex morphology / MGI
  • cortical renal glomerulopathies / MGI
  • kidney hemorrhage / MGI
  • decreased body size / MGI
  • abnormal renal tubule morphology / MGI
  • dilated renal tubules / MGI
  • abnormal renal glomerular capsule morphology / MGI
  • albuminuria / MGI
  • increased urine protein level / MGI
  • kidney failure / MGI
  • abnormal proximal convoluted tubule morphology / MGI
  • glomerulosclerosis / MGI
  • abnormal renal glomerulus morphology / MGI
  • abnormal podocyte morphology / MGI
  • renal/urinary system phenotype / MGI
  • increased circulating creatinine level / MGI
  • increased blood urea nitrogen level / MGI
  • abnormal podocyte foot process morphology / MGI
  • absent podocyte slit diaphragm / MGI
  • podocyte foot process effacement / MGI
  • dilated proximal convoluted tubules / MGI
  • abnormal renal tubule epithelium morphology / MGI
  • postnatal lethality, complete penetrance / MGI
  • perinatal lethality, incomplete penetrance / MGI
  • renal glomerulus hypertrophy / MGI
  • abnormal kidney arterial blood vessel morphology / MGI
  • abnormal glomerular capillary morphology / MGI
  • abnormal peritubular capillary morphology / MGI
  • abnormal renal glomerulus basement membrane morphology / MGI
  • expanded mesangial matrix / MGI
  • renal cast / MGI
  • mesangiolysis / MGI
  • abnormal glomerular capillary endothelium morphology / MGI
  • abnormal glomerular endothelium fenestra morphology / MGI
  • glomerular crescent / MGI
  • dilated glomerular capillary / MGI
  • podocyte microvillus transformation / MGI
  • podocyte hypertrophy / MGI

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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