C57BL/6N-Spryd3tm1b(EUCOMM)Hmgu/Ieg

Status

Available to order

EMMA IDEM:10031
International strain nameC57BL/6N-Spryd3tm1b(EUCOMM)Hmgu/Ieg
Alternative nameHEPD0539_9_C10
Strain typeTargeted Mutant Strains
Allele/Transgene symbolSpryd3tm1b(EUCOMM)Hmgu
Gene/Transgene symbolSpryd3
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliationInstitute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic informationThis mouse line originates from EUCOMM ES clone HEPD0539_9_C10. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele (Gt(ROSA)26Sor). Click here for more information on EUCOMM final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivingheterozygous C57BL/6NTac

Disease and phenotype information

IMPC phenotypes (allele matching)
  • abnormal lens morphology / IMPC
  • cataract / IMPC
  • abnormal retina morphology / IMPC
  • increased startle reflex / IMPC
  • increased circulating HDL cholesterol level / IMPC
  • increased circulating insulin level / IMPC
  • abnormal vitreous body morphology / IMPC
  • abnormal retina vasculature morphology / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • increased circulating cholesterol level / IMPC
  • increased circulating creatinine level / IMPC
  • abnormal retina blood vessel morphology / IMPC
IMPC phenotypes (gene matching)
  • abnormal lens morphology / IMPC
  • abnormal retina blood vessel morphology / IMPC
  • abnormal retina vasculature morphology / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • increased startle reflex / IMPC
  • increased circulating insulin level / IMPC
  • increased grip strength / IMPC
  • abnormal retina morphology / IMPC
  • abnormal vitreous body morphology / IMPC
  • increased circulating HDL cholesterol level / IMPC
  • increased circulating creatinine level / IMPC
  • increased circulating cholesterol level / IMPC
  • cataract / IMPC
  • increased circulating aspartate transaminase level / IMPC

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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