B6;129P2-Wnt4tm2(EGFP/cre)Svo/Oulu

Status

Available to order

EMMA IDEM:10011
Citation informationRRID:IMSR_EM:10011 

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International strain nameB6;129P2-Wnt4tm2(EGFP/cre)Svo/Oulu
Alternative nameWnt-4-Cre
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolWnt4tm2(EGFP/cre)Svo
Gene/Transgene symbolWnt4

Information from provider

ProviderJingdong Shan
Provider affiliationDepartment of Biochemistry and Molecular Medicine, University of Oulu
Additional ownerDr. Seppo Vainio, University of Oulu, Oulu, Finland
Genetic informationTargeted construct in 129/Ola ES cells replaces the Wnt4 gene after the promoter with cre recombinase.
Phenotypic informationHomozygous:
Wnt4-cre homozygotes have the same phenotype as Wnt4 knockouts. Kidney malformation and female to male sex reversal. Homozygotes die few days after birth.

Heterozygous:
Heterozygous mice have no reported phenotypes.
Breeding historyTargeted mutation was directly maintained on a C57BL/6 background.
References
  • Generation of an allele to inactivate Wnt4 gene function conditionally in the mouse.;Shan Jingdong, Jokela Tiina, Peltoketo Hellevi, Vainio Seppo, ;2009;Genesis (New York, N.Y. : 2000);47;782-8; 19830824
  • Mapping of the fate of cell lineages generated from cells that express the Wnt4 gene by time-lapse during kidney development.;Shan Jingdong, Jokela Tiina, Skovorodkin Ilya, Vainio Seppo, ;2010;Differentiation; research in biological diversity;79;57-64; 19740593
Homozygous fertilenot known
Homozygous viableno
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreUniversity of Oulu, Oulu, Finland
Animals used for archivingheterozygous C57BL/6JOlaHsd males, wild-type C57BL/6NCrl females
Stage of embryos2-cell

Disease and phenotype information

MGI phenotypes (gene matching)
  • absent kidney / MGI
  • abnormal kidney development / MGI
  • delayed kidney development / MGI
  • decreased thymocyte number / MGI
  • abnormal ovary morphology / MGI
  • abnormal oogenesis / MGI
  • secondary sex reversal / MGI
  • no abnormal phenotype detected / MGI
  • abnormal secondary sex determination / MGI
  • abnormal adrenal gland physiology / MGI
  • no phenotypic analysis / MGI
  • kidney failure / MGI
  • nervous system phenotype / MGI
  • abnormal reproductive system development / MGI
  • abnormal adenohypophysis morphology / MGI
  • decreased renal glomerulus number / MGI
  • decreased oocyte number / MGI
  • abnormal female germ cell morphology / MGI
  • absent Mullerian ducts / MGI
  • decreased somatotroph cell number / MGI
  • decreased gonadotroph cell number / MGI
  • decreased thyrotroph cell number / MGI
  • oocyte degeneration / MGI
  • abnormal metanephric mesenchyme morphology / MGI
  • abnormal nephrogenic mesenchyme morphogenesis / MGI
  • postnatal lethality, complete penetrance / MGI
  • neonatal lethality, complete penetrance / MGI
  • small metanephros / MGI

Literature references

  • Generation of an allele to inactivate Wnt4 gene function conditionally in the mouse.;Shan Jingdong, Jokela Tiina, Peltoketo Hellevi, Vainio Seppo, ;2009;Genesis (New York, N.Y. : 2000);47;782-8; 19830824
  • Mapping of the fate of cell lineages generated from cells that express the Wnt4 gene by time-lapse during kidney development.;Shan Jingdong, Jokela Tiina, Skovorodkin Ilya, Vainio Seppo, ;2010;Differentiation; research in biological diversity;79;57-64; 19740593

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

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Legally binding conditions for the transfer

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