Service Description:

The Animal Modelling Facility (AMF) at the Netherlands Cancer Institute (NKI) provides all services related to the generation of genetically engineered mice (GEM) based on zygote manipulation by CRISPR/Cas9 procedures, including full-service covering the design and all hands-on steps. The AMF keeps track of recent technological developments and tests their usefulness for improving model generation. These include the application of CRISPR/Cas technologies in combination with Adeno Associated Viral vectors for the targeted introduction of larger inserts up to 4kbp.

Included in the Service:

After consultation with the researcher the AMF designs the genetic modification strategy including the setup of efficient genotyping protocols. Using the first generation of mice (founder) carrying the desired modification the AMF will set up breeding procedures to obtain the F1 generation to establish the genetically modified strain the for delivery to the researcher and long term cryopreservation. If needed the AMF will also enable revitalisation of the strains.

Additional Support:

The AMF provides the services of modulating ES-cells for in vitro studies and produce viruses for somatic modelling.

How to apply for the INFRAFRONTIER Mouse Model Generation service?

Please click the button below to fill out our Service Request Form or Pbagnpg hf if you have any questions.

If you have a preferred service provider, please indicate it in the form.

Request Our Service Here

Get to know the provider:

The Animal Modelling Facility (AMF) at the Netherlands Cancer Institute (NKI) consists of a fully equipped molecular biology facility for all the procedures involving assembly and validation by sequencing of DNA constructs involved in each zygote embryo manipulation. Also, genotyping of the mice derived from embryo manipulation and transfer is performed in this facility. In the Animal Facility the AMF has 1 room for embryo manipulation including 2 tissue culture incubators and 4 embryo manipulation stages. In addition, the AMF has 2 animal rooms in which mice are housed in individually ventilated cages at SOPF conditions.

Learn more about NKI here.

Relevant Publications

  1. McLelland GL, Lopez-Osias M, Verzijl CRC, et al. Identification of an alternative triglyceride biosynthesis pathway. Nature. 2023; 621(7977):171-178 doi.org/10.1038/s41586-023-06497-4

  2. Gulen MF, Samson N, Keller A, et al. cGAS-STING drives ageing-related inflammation and neurodegeneration. Nature. 2023; 620(7973):374-380 doi.org/10.1038/s41586-023-06373-1

  3. Morgner J, Bornes L, Hahn K, et al. A Lamb1Dendra2 mouse model identifies basement-membrane-producing origins and dynamics in PyMT breast tumors. Dev Cell. 2023;58(7):535-549 doi.org/10.1016/j.devcel.2023.02.017

INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).