Service Description:

The Mouse Embryo Cryopreservation Facility at the National Centre for Biotechnology (CNB-CSIC) offers the generation of genetically edited mice with CRISPR/Cas9 tools.

Included in the Service:

The generation of new mouse models genetically edited with CRISPR/Cas9 tools in mouse embryos will be carried out by electroporation. The new mouse models will be generated and deposited in the INFRAFRONTIER/EMMA repository. The interested user will request access to these mice using the INFRAFRONTIER/EMMA standard procedures of requesting existing cryopreserved mouse lines. The following types of mutated alleles will be produced: gene inactivation; deletions up to ~10 Kb, and addition or substitution (indels) up to ~5 bp with template oligonucleotides. The provision of mouse models generated with CRISPR will be accomplished through INFRAFRONTIER/EMMA.

CRISPR activities of INFRAFRONTIER/EMMA are covered by non-exclusive worldwide licenses granted by Caribou Biosciences, Inc. and the Broad Institute, Inc. to Helmholtz Zentrum München – Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH). Researchers are entitled to request up to one year (for CRISPR mouse strains) of no visibility for their mouse models deposited into the INFRAFRONTIER/EMMA database and the detailed license conditions can be found in the applicable Material Transfer Agreement (MTA).

Additional Support:

The CNB Mouse Embryo Cryopreservation Facility can provide additional support for the design of reagents (RNA guides, DNA primers, type of Cas9 reagents) and for the best strategy to be applied in each case, for each new mouse model to be generated using the CRISPR/Cas9 technology.