Service Description:

The Czech Centre for Phenogenomics (CCP) offers Model Generation Service that includes zygote electroporation of CRISPR/Cas9-based targeting tools, pronuclear microinjection of DNA constructs into mouse zygotes, microinjection of targeted ES cell lines into morulas (8-stage cell embryo) to produce chimeric mice; mouse archiving (cryopreservation of embryos and sperm); and recovery of live mice from cryopreserved embryos and sperm, analysis of sperm viability, rederivation of mouse strains and lines, and others. CCP also provides consultation and assistance services, and information on the design and use of genetically modified transgenic mice.

Additional Support:

Phenotyping:
CCP provides a comprehensive collection of tools for the physiological and morphological assessment of experimental mice and rats in a controlled SPF (specific pathogen-free) environment.

Animal Facility:
CCP provides an optimal SPF breeding condition for small laboratory rodents with the total capacity approximately 24 000 animals. Animal facility also provides complete service under BSL-3 conditions.

Preclinical testing:
CCP can prepare and proceed projects from a broad variety of physiological categories from single parameter analysis (for example glucose level) to multi parametric analysis using panels from clinical biochemistry, immunology, cytokine levels etc.