C57BL/6N-A^tm1Brd Acer1^{tm1a(EUCOMM)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:08275
International strain name	C57BL/6N-A^tm1Brd Acer1^{tm1a(EUCOMM)Wtsi}/WtsiH
Alternative name	EPD0377_3_B06
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Acer1^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Acer1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0377_3_B06. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Alkaline ceramidase 1 is essential for mammalian skin homeostasis and regulating whole-body energy expenditure.;Liakath-Ali Kifayathullah, Vancollie Valerie E, Lelliott Christopher J, Speak Anneliese O, Lafont David, Protheroe Hayley J, Ingvorsen Camilla, Galli Antonella, Green Angela, Gleeson Diane, Ryder Ed, Glover Leanne, Vizcay-Barrena Gema, Karp Natasha A, Arends Mark J, Brenn Thomas, Spiegel Sarah, Adams David J, Watt Fiona M, van der Weyden Louise, ;2016;The Journal of pathology;239;374-83; 27126290

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (allele matching)

abnormal retina morphology / IMPC
abnormal hair growth / IMPC

IMPC phenotypes (gene matching)

abnormal hair growth / IMPC
abnormal retina morphology / IMPC

MGI phenotypes (allele matching)

abnormal coat/ hair morphology / MGI
abnormal hair follicle morphology / MGI
abnormal awl hair morphology / MGI
abnormal zigzag hair morphology / MGI
alopecia / MGI
enlarged sebaceous gland / MGI
scaly skin / MGI
epidermal hyperplasia / MGI
abnormal epidermis stratum granulosum morphology / MGI
abnormal epidermis stratum corneum morphology / MGI
hyperkeratosis / MGI
thick dermal layer / MGI
decreased body weight / MGI
hyperactivity / MGI
abnormal lipid level / MGI
abnormal hair growth / MGI
impaired skin barrier function / MGI
abnormal hair follicle development / MGI
abnormal hair shaft morphology / MGI
abnormal hair cuticle / MGI
dry skin / MGI
increased energy expenditure / MGI
skin inflammation / MGI
increased eosinophil cell number / MGI
increased apoptosis / MGI
increased basal metabolism / MGI
decreased brown fat cell lipid droplet size / MGI
increased epididymal fat pad weight / MGI
decreased epididymal fat pad weight / MGI
decreased inguinal fat pad weight / MGI
delayed skin barrier formation / MGI
abnormal skin sebaceous gland morphology / MGI
increased keratinocyte proliferation / MGI
abnormal keratohyalin granule morphology / MGI
abnormal hair follicle bulge morphology / MGI
abnormal sphingomyelin level / MGI
abnormal hair follicle infundibulum morphology / MGI
dilated hair follicle infundibulum / MGI
increased food intake / MGI
abnormal ceramide level / MGI
abnormal sebocyte morphology / MGI
decreased brown adipose tissue mass / MGI
decreased sphingosine level / MGI

MGI phenotypes (gene matching)

abnormal coat/ hair morphology / MGI
abnormal hair follicle morphology / MGI
abnormal awl hair morphology / MGI
abnormal zigzag hair morphology / MGI
alopecia / MGI
abnormal hair cycle / MGI
enlarged sebaceous gland / MGI
scaly skin / MGI
epidermal hyperplasia / MGI
abnormal epidermis stratum granulosum morphology / MGI
abnormal epidermis stratum corneum morphology / MGI
hyperkeratosis / MGI
thick dermal layer / MGI
decreased body weight / MGI
weight loss / MGI
hyperactivity / MGI
abnormal lipid level / MGI
abnormal hair growth / MGI
impaired skin barrier function / MGI
abnormal sebaceous lipid secretion / MGI
abnormal hair follicle development / MGI
abnormal hair shaft morphology / MGI
abnormal hair cuticle / MGI
dry skin / MGI
abnormal skin development / MGI
increased energy expenditure / MGI
skin inflammation / MGI
increased eosinophil cell number / MGI
abnormal skin physiology / MGI
abnormal enzyme/coenzyme activity / MGI
increased apoptosis / MGI
abnormal hair cycle anagen phase / MGI
abnormal hair cycle telogen phase / MGI
increased basal metabolism / MGI
decreased brown fat cell lipid droplet size / MGI
increased epididymal fat pad weight / MGI
decreased epididymal fat pad weight / MGI
decreased inguinal fat pad weight / MGI
delayed skin barrier formation / MGI
abnormal skin sebaceous gland morphology / MGI
increased keratinocyte proliferation / MGI
abnormal keratohyalin granule morphology / MGI
abnormal hair follicle bulge morphology / MGI
sebaceous gland hypertrophy / MGI
abnormal sphingomyelin level / MGI
abnormal hair follicle infundibulum morphology / MGI
dilated hair follicle infundibulum / MGI
mortality/aging / MGI
abnormal hair follicle physiology / MGI
increased food intake / MGI
abnormal ceramide level / MGI
abnormal sebocyte morphology / MGI
decreased brown adipose tissue mass / MGI
decreased sphingosine level / MGI
abnormal piliary canal morphology / MGI

Literature references

Alkaline ceramidase 1 is essential for mammalian skin homeostasis and regulating whole-body energy expenditure.;Liakath-Ali Kifayathullah, Vancollie Valerie E, Lelliott Christopher J, Speak Anneliese O, Lafont David, Protheroe Hayley J, Ingvorsen Camilla, Galli Antonella, Green Angela, Gleeson Diane, Ryder Ed, Glover Leanne, Vizcay-Barrena Gema, Karp Natasha A, Arends Mark J, Brenn Thomas, Spiegel Sarah, Adams David J, Watt Fiona M, van der Weyden Louise, ;2016;The Journal of pathology;239;374-83; 27126290

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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