B6;Cg-Nrp1tm1.1Cruh/H
Status | Available to order |
EMMA ID | EM:11987 |
International strain name | B6;Cg-Nrp1tm1.1Cruh/H |
Alternative name | Nrp1cyto |
Strain type | Targeted Mutant Strains : Knock-in |
Allele/Transgene symbol | Nrp1tm1.1Cruh, |
Gene/Transgene symbol | Nrp1 |
Information from provider
Provider | Christiana Ruhrberg |
Provider affiliation | UCL Institute of Ophthalmology, University College London |
Genetic information | A 4 base-pair insertion (AATT) into the Nrp1 last exon after the transmembrane domain coding sequence, shifting the reading frame and preventing translation of the cytoplasmic domain of Nrp1. |
Phenotypic information | Homozygous:The analysis of pre- and perinatal vascular development revealed that vasculogenesis and angiogenesis proceed normally in these mutants, demonstrating that the Nrp1 membrane-anchored extracellular domain is sufficient for vessel growth. By contrast, the cytoplasmic domain is required for normal arteriovenous patterning, because arteries and veins crossed each other at an abnormally high frequency in the Nrp1-cyto delta/delta retina. At crossing sites, the artery was positioned anteriorly to the vein, and both vessels were embedded in a shared collagen sleeve.Heterozygous:-- |
References |
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Homozygous fertile | yes |
Homozygous viable | yes |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
MGI allele-associated human disease models
Literature references
- The cytoplasmic domain of neuropilin 1 is dispensable for angiogenesis, but promotes the spatial separation of retinal arteries and veins.;Fantin Alessandro, Schwarz Quenten, Davidson Kathryn, Normando Eduardo M, Denti Laura, Ruhrberg Christiana, ;2011;Development (Cambridge, England);138;4185-91; 21852397
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