B6N.129P2(Cg)-Stat1tm2.1Bstr/Biat
Status | Available to order |
EMMA ID | EM:09915 |
International strain name | B6N.129P2(Cg)-Stat1tm2.1Bstr/Biat |
Alternative name | B6.129P2-Stat1alpha |
Strain type | Targeted Mutant Strains : Knock-in |
Allele/Transgene symbol | Stat1tm2.1Bstr, |
Gene/Transgene symbol | Stat1 |
Information from provider
Provider | Mathias Müller |
Provider affiliation | Department for Biomedical Sciences, University of Veterinary Medicine Vienna (Vetmeduni Vienna) |
Additional owner | Prof. Thomas Rülicke, University of Veterinary Medicine, Institute of Laboratory Animal Science, Vienna, Austria |
Genetic information | The terminal portion of the cDNA for Stat1beta fused in frame into exon 19 of the genomic Stat1 gene (accession no. AF349678). A human beta-globin splice and a floxed neomycin cassette were inserted downstream of the cDNAs and placed between two regions of homology. Offspring carrying the targeted alleles were crossed to Tg(CMV-cre) mice (Schwenk et al. 1995 Nucleic Acids Res. 23:5080–5081) to remove the neomycin cassette and subsequently intercrossed to obtain Stat1 beta/beta homozygous mice. |
Phenotypic information | Homozygous:Stat1 beta/beta mice show more prolonged IFN-gamma-induced phosphorylation and promoter binding; mediates protective, IFN-gamma-dependent immunity against the bacterium Listeria monocytogenes.Heterozygous:n/a |
Breeding history | The mice were backcrossed to the C57BL/6N background by speed congenics (Teppner et al. 2004; Lab Anim. 38:406–412. doi:10.1258/0023677041958882). |
References |
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Homozygous fertile | yes |
Homozygous viable | yes |
Homozygous matings required | no |
Immunocompromised | not known |
Information from EMMA
Archiving centre | University of Veterinary Medicine, Vienna, Austria |
Animals used for archiving | homozygous C57BL/6.Cg |
Literature references
- STAT1β is not dominant negative and is capable of contributing to gamma interferon-dependent innate immunity.;Semper Christian, Leitner Nicole R, Lassnig Caroline, Parrini Matthias, Mahlakõiv Tanel, Rammerstorfer Michael, Lorenz Karin, Rigler Doris, Müller Simone, Kolbe Thomas, Vogl Claus, Rülicke Thomas, Staeheli Peter, Decker Thomas, Müller Mathias, Strobl Birgit, ;2014;Molecular and cellular biology;34;2235-48; 24710278
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