CD1.129S-Stat1+/Cnbc
Status | Available to order |
EMMA ID | EM:06064 |
International strain name | CD1.129S-Stat1+/Cnbc |
Alternative name | CD1.129-Stat1<+/+>-Tg(APN)270<-/->/Cnbc |
Strain type | Targeted Mutant Strains : Other targeted |
Allele/Transgene symbol | Stat1+, |
Gene/Transgene symbol | Stat1 |
Information from provider
Provider | Luis Enjuanes |
Provider affiliation | Centro Nacional de Biotecnologia (CNB-CSIC) |
Additional owner | There are 14 strains of Stat1 KO available through IMSR, including the one used here from David Levy's lab at NYU (Stat1 |
Genetic information | Transgenic mice were produced in ICR (CD1) mouse outbred background by standard pronuclear microinjection and bred to homozygosity. Stat1 KO mice were produced and donated by David Levy (NYU) in 129S/Sv (CCE ES cells) background and backcrossed to CD1 mouse background to homozygosity. A neomycin resistance cassette replaced 5.7 kb of sequence, including 3 exons and a portion of a fourth encoding amino acids 221-365. This allele produces a partial non-functional protein product. Both mouse lines were bred to double homozygosity in CD1 background. Line 270 and 861 expressed the highest levels of APN. These mice are double homozygous for non-transgenic line APN (wild-type) and double homozygous for Stat1 wild-type allele. |
Phenotypic information | Control mice for the entire experiment. |
Breeding history | Stat1 KO mice originally generated in 129S/Sv background (CCE ES cells) and backcrossed to CD1. Tg APN mice generated in CD1(ICR) mice. Both mouse lines bred to double homozygous line. In this case Stat1 locus carries both wild-type alleles and there is no APN transgene. |
References |
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Homozygous fertile | yes |
Homozygous viable | yes |
Homozygous matings required | yes |
Immunocompromised | no |
Information from EMMA
Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Autoimmune enteropathy and endocrinopathy-susceptibility to chronic infections syndrome / Orphanet_391487
- Susceptibility to viral and mycobacterial infections due to STAT1 deficiency / Orphanet_391311
- Mendelian susceptibility to mycobacterial diseases due to partial STAT1 deficiency / Orphanet_319595
Literature references
- Development of a transgenic mouse model susceptible to human coronavirus 229E.;Lassnig Caroline, Sanchez Carlos M, Egerbacher Monika, Walter Ingrid, Majer Susanne, Kolbe Thomas, Pallares Pilar, Enjuanes Luis, Müller Mathias, ;2005;Proceedings of the National Academy of Sciences of the United States of America;102;8275-80; 15919828
- Targeted disruption of the mouse Stat1 gene results in compromised innate immunity to viral disease.;Durbin J E, Hackenmiller R, Simon M C, Levy D E, ;1996;Cell;84;443-50; 8608598
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