IMPC phenotypes (gene matching)
B6.129X1(129S4)-Il6tm1.1Jho/Cnbc
| Status | Available to order |
| EMMA ID | EM:05332 |
| International strain name | B6.129X1(129S4)-Il6tm1.1Jho/Cnbc |
| Alternative name | Interleukin-6 floxed mice |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Il6tm1.1Jho |
| Gene/Transgene symbol | Il6 |
Information from provider
| Provider | Juan Hidalgo |
| Provider affiliation | Biología Celular, Fisiología e Inmunología, Universidad Autónoma de Barcelona |
| Genetic information | Floxed mouse line for interleukin 6 (Il6). To this end, exon 2 from Il6 gene has been flanked with loxP sites. |
| Phenotypic information | With this number of backcrosses (ca. 5) the floxed mice show some phenotype, since the floxed Il6 gene is expressed less than in normal wild-type mice upon endotoxin administration. We have already produced some conditional KO mice with several cre-expressing mouse lines and there is a clear difference between floxed and KO mice regarding Il6 levels. |
| Breeding history | Neo cassette was removed by crossing with flp-expressing mice once one of the chimeras was found to have germ line transmission upon crossing with C57BL/6. The flp gene was removed upon crossing with C57BL/6. Finally, offspring mice positive for the floxed gene were crossed with each other and a homozygous line of these floxed mice is maintained that way in the general animal services of this university. We have started to further backcross this line with C57BL/6 in the Animal Physiology unit; in total we are about in the fifth generation. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | yes |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6JOlaHsd, wild-type C57BL/6JOlaHsd |
| Stage of embryos | 2-cell |
Disease and phenotype information
MGI phenotypes (gene matching)
- decreased bone mineral density / MGI
- abnormal trabecular bone morphology / MGI
- decreased compact bone thickness / MGI
- abnormal microglial cell morphology / MGI
- abnormal heart morphology / MGI
- abnormal heart development / MGI
- thin ventricular wall / MGI
- abnormal interventricular septum morphology / MGI
- abnormal liver morphology / MGI
- abnormal lymphocyte cell number / MGI
- abnormal CNS glial cell morphology / MGI
- abnormal body weight / MGI
- obese / MGI
- decreased body weight / MGI
- increased aggression towards males / MGI
- abnormal social investigation / MGI
- increased anxiety-related response / MGI
- abnormal stationary movement / MGI
- hyperactivity / MGI
- abnormal object recognition memory / MGI
- abnormal spatial learning / MGI
- abnormal sleep pattern / MGI
- abnormal osteoclast physiology / MGI
- abnormal cardiovascular system physiology / MGI
- hepatic necrosis / MGI
- abnormal homeostasis / MGI
- abnormal immune system physiology / MGI
- impaired wound healing / MGI
- decreased IgA level / MGI
- abnormal inflammatory response / MGI
- brain inflammation / MGI
- decreased inflammatory response / MGI
- abnormal dopamine level / MGI
- decreased chemically-elicited antinociception / MGI
- decreased incidence of induced tumors / MGI
- abnormal learning/memory/conditioning / MGI
- seizures / MGI
- abnormal glucose homeostasis / MGI
- abnormal T cell differentiation / MGI
- abnormal astrocyte morphology / MGI
- gliosis / MGI
- abnormal exercise endurance / MGI
- abnormal pulmonary gas exchange / MGI
- increased susceptibility to bacterial infection / MGI
- increased susceptibility to viral infection / MGI
- abnormal T-helper 1 cell morphology / MGI
- abnormal T cell physiology / MGI
- abnormal macrophage physiology / MGI
- abnormal neutrophil physiology / MGI
- decreased IgE level / MGI
- abnormal emotion/affect behavior / MGI
- behavioral despair / MGI
- decreased glucagon secretion / MGI
- abnormal mechanical nociception / MGI
- dilated heart left ventricle / MGI
- abnormal passive avoidance behavior / MGI
- abnormal long term object recognition memory / MGI
- decreased heart weight / MGI
- abnormal neuron morphology / MGI
- delayed wound healing / MGI
- abnormal bone remodeling / MGI
- abnormal bone healing / MGI
- decreased insulin secretion / MGI
- abnormal skeletal muscle fiber morphology / MGI
- abnormal muscle precursor cell migration / MGI
- liver degeneration / MGI
- abnormal frequency of paradoxical sleep / MGI
- liver failure / MGI
- decreased susceptibility to induced arthritis / MGI
- increased fear-related response / MGI
- abnormal nervous system physiology / MGI
- abnormal glial cell morphology / MGI
- abnormal capillary morphology / MGI
- abnormal microglial cell physiology / MGI
- impaired febrile response / MGI
- abnormal brain interneuron morphology / MGI
- decreased incidence of tumors by chemical induction / MGI
- abnormal neuron physiology / MGI
- abnormal behavior / MGI
- decreased T cell number / MGI
- increased susceptibility to parasitic infection / MGI
- decreased cytotoxic T cell cytolysis / MGI
- decreased cardiac muscle contractility / MGI
- abnormal B cell proliferation / MGI
- decreased susceptibility to injury / MGI
- abnormal oxygen consumption / MGI
- impaired glucose tolerance / MGI
- abnormal heart ventricle morphology / MGI
- homeostasis/metabolism phenotype / MGI
- growth/size/body region phenotype / MGI
- behavior/neurological phenotype / MGI
- immune system phenotype / MGI
- increased susceptibility to fungal infection / MGI
- abnormal axon morphology / MGI
- abnormal food intake / MGI
- abnormal T-helper 1 physiology / MGI
- abnormal T-helper 2 physiology / MGI
- decreased liver regeneration / MGI
- increased body temperature / MGI
- increased circulating glucose level / MGI
- decreased dopamine level / MGI
- abnormal involution of the mammary gland / MGI
- absent NK cells / MGI
- decreased pro-B cell number / MGI
- abnormal osteoblast differentiation / MGI
- abnormal osteoclast differentiation / MGI
- increased IgG2a level / MGI
- retinal outer nuclear layer degeneration / MGI
- increased susceptibility to induced colitis / MGI
- decreased interferon-gamma secretion / MGI
- increased circulating interleukin-10 level / MGI
- decreased circulating interleukin-12 level / MGI
- decreased interleukin-17 secretion / MGI
- increased interleukin-4 secretion / MGI
- abnormal interleukin-6 secretion / MGI
- impaired neutrophil recruitment / MGI
- abnormal interferon level / MGI
- abnormal skeletal muscle satellite cell proliferation / MGI
- enhanced behavioral response to morphine / MGI
- increased sensitivity to induced morbidity/mortality / MGI
- increased susceptibility to bacterial infection induced morbidity/mortality / MGI
- abnormal oval cell physiology / MGI
- abnormal bone mineral density / MGI
- abnormal acute phase protein level / MGI
- mortality/aging / MGI
- increased susceptibility to dopaminergic neuron neurotoxicity / MGI
- abnormal substantia nigra pars compacta morphology / MGI
- abnormal inhibitory learning / MGI
- abnormal pancreatic alpha cell mass / MGI
Literature references
- Astrocyte-specific deficiency of interleukin-6 and its receptor reveal specific roles in survival, body weight and behavior.;Quintana Albert, Erta Maria, Ferrer Beatriz, Comes Gemma, Giralt Mercedes, Hidalgo Juan, ;2013;Brain, behavior, and immunity;27;162-73; 23085146
- Identification of liver cancer progenitors whose malignant progression depends on autocrine IL-6 signaling.;He Guobin, Dhar Debanjan, Nakagawa Hayato, Font-Burgada Joan, Ogata Hisanobu, Jiang Yuhong, Shalapour Shabnam, Seki Ekihiro, Yost Shawn E, Jepsen Kristen, Frazer Kelly A, Harismendy Olivier, Hatziapostolou Maria, Iliopoulos Dimitrios, Suetsugu Atsushi, Hoffman Robert M, Tateishi Ryosuke, Koike Kazuhiko, Karin Michael, ;2013;Cell;155;384-96; 24120137
- MHC class II-dependent B cell APC function is required for induction of CNS autoimmunity independent of myelin-specific antibodies.;Molnarfi Nicolas, Schulze-Topphoff Ulf, Weber Martin S, Patarroyo Juan C, Prod'homme Thomas, Varrin-Doyer Michel, Shetty Aparna, Linington Christopher, Slavin Anthony J, Hidalgo Juan, Jenne Dieter E, Wekerle Hartmut, Sobel Raymond A, Bernard Claude C A, Shlomchik Mark J, Zamvil Scott S, ;2013;The Journal of experimental medicine;210;2921-37; 24323356
- Interleukin-6 (IL-6) deficiency enhances intramembranous osteogenesis following stress fracture in mice.;Coates Brandon A, McKenzie Jennifer A, Yoneda Susumu, Silva Matthew J, ;2021;Bone;143;115737; 33181349