IMPC phenotypes (gene matching)
- abnormal posture / IMPC
B6;129P2-Gt(ROSA)26Sortm1(CAG-Venus/GMNN,-Cherry/CDT1)Jkn/JknH
| Status | Available to order |
| EMMA ID | EM:08395 |
| International strain name | B6;129P2-Gt(ROSA)26Sortm1(CAG-Venus/GMNN,-Cherry/CDT1)Jkn/JknH |
| Alternative name | R26Fucci2aR |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Gt(ROSA)26Sortm1(CAG-Venus/GMNN,Cherry/CDT1)Jkn |
| Gene/Transgene symbol | Gt(ROSA)26Sor |
Information from provider
| Provider | Richard Mort |
| Provider affiliation | Department of Medical & Developmental Genetics, MRC Human Genetics Unit |
| Genetic information | A targeted insertion of CAG-floxNeo-Fucci2a-pA into the Gt(ROSA)26Sor locus on mouse chromosome 6. |
| Phenotypic information | The Fucci system was developed by fusing red- and green-emitting fluorescent proteins to the E3 ligase substrates, Cdt1 (chromatin licensing and DNA replication factor 1) and geminin. These fluorescent probes make it possible to distinguish between cells in the G1 or S/G2/M phases of the cell cycle, in live cells/tissue (Sakaue-Sawano A. et al., 2008). Fucci2a is a bicistronic version of Fucci2 allowing cre recombinase-mediated tissue-specific expression in mice (Mort R. et al., 2014). |
| Breeding history | Targeted in E14 129P2/Ola ES cells. Subsequently backcrossed for ca. 3 generations onto C57BL/6. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
MGI phenotypes (gene matching)
- abnormal ear pigmentation / MGI
- decreased circulating LDL cholesterol level / MGI
- decreased hematocrit / MGI
- increased leukocyte cell number / MGI
- decreased neutrophil cell number / MGI
- extramedullary hematopoiesis / MGI
- abnormal erythropoiesis / MGI
- abnormal myocardial fiber morphology / MGI
- abnormal cell death / MGI
- increased granulocyte number / MGI
- increased cell proliferation / MGI
- alopecia / MGI
- short snout / MGI
- abnormal digestive system morphology / MGI
- gastrointestinal hemorrhage / MGI
- increased rib number / MGI
- abnormal pulmonary artery morphology / MGI
- rectal prolapse / MGI
- abnormal renal/urinary system morphology / MGI
- abnormal mammary gland development / MGI
- abnormal spleen morphology / MGI
- enlarged spleen / MGI
- enlarged lymph nodes / MGI
- dystrophic muscle / MGI
- absent notochord / MGI
- abnormal retinal photoreceptor morphology / MGI
- abnormal neuromuscular synapse morphology / MGI
- pigmentation phenotype / MGI
- increased body weight / MGI
- decreased body weight / MGI
- decreased body size / MGI
- retinal degeneration / MGI
- disorganized retinal layers / MGI
- abnormal optic nerve morphology / MGI
- decreased anxiety-related response / MGI
- circling / MGI
- hypoactivity / MGI
- impaired coordination / MGI
- abnormal eating behavior / MGI
- limb grasping / MGI
- increased circulating triglyceride level / MGI
- increased circulating free fatty acid level / MGI
- increased circulating HDL cholesterol level / MGI
- hyperglycemia / MGI
- anemia / MGI
- abnormal blood vessel morphology / MGI
- abnormal mesoderm development / MGI
- abnormal somite development / MGI
- decreased trophoblast giant cell number / MGI
- postnatal growth retardation / MGI
- impaired wound healing / MGI
- abnormal humoral immune response / MGI
- arrested B cell differentiation / MGI
- arrested T cell differentiation / MGI
- increased inflammatory response / MGI
- reduced fertility / MGI
- reduced female fertility / MGI
- male infertility / MGI
- decreased litter size / MGI
- respiratory distress / MGI
- abnormal pain threshold / MGI
- increased thermal nociceptive threshold / MGI
- abnormal coat/hair pigmentation / MGI
- abnormal glucose homeostasis / MGI
- increased circulating insulin level / MGI
- premature death / MGI
- abnormal tail morphology / MGI
- abnormal kidney morphology / MGI
- abnormal neural tube morphology / MGI
- no abnormal phenotype detected / MGI
- abnormal seminiferous tubule morphology / MGI
- small lymph nodes / MGI
- abnormal spleen white pulp morphology / MGI
- abnormal bone marrow cell morphology/development / MGI
- abnormal megakaryocyte progenitor cell morphology / MGI
- abnormal proerythroblast morphology / MGI
- abnormal megakaryocyte morphology / MGI
- increased mean corpuscular volume / MGI
- decreased mean corpuscular volume / MGI
- increased mean platelet volume / MGI
- hepatic steatosis / MGI
- abnormal epididymis morphology / MGI
- vestigial tail / MGI
- reticulocytosis / MGI
- abnormal keratinocyte differentiation / MGI
- decreased circulating corticosterone level / MGI
- asthenozoospermia / MGI
- oligozoospermia / MGI
- abnormal renal tubule morphology / MGI
- decreased circulating insulin level / MGI
- abnormal chemical nociception / MGI
- dilated heart left ventricle / MGI
- decreased vertical activity / MGI
- abnormal notochord morphology / MGI
- abnormal ocular fundus morphology / MGI
- albuminuria / MGI
- decreased hemoglobin content / MGI
- decreased erythrocyte cell number / MGI
- no phenotypic analysis / MGI
- vertebral transformation / MGI
- abnormal lumbar vertebrae morphology / MGI
- abnormal skeletal muscle fiber morphology / MGI
- absent allantois / MGI
- hypospadia / MGI
- anal atresia / MGI
- increased erythroid progenitor cell number / MGI
- abnormal embryonic hematopoiesis / MGI
- kinked neural tube / MGI
- epididymal inflammation / MGI
- nervous system phenotype / MGI
- abnormal nervous system morphology / MGI
- kidney cysts / MGI
- pallor / MGI
- abnormal neural tube closure / MGI
- abnormal retinal inner nuclear layer morphology / MGI
- vascular smooth muscle hypoplasia / MGI
- skeletal muscle necrosis / MGI
- abnormal defecation / MGI
- abnormal heart left ventricle morphology / MGI
- abnormal circulating lipid level / MGI
- abnormal hormone level / MGI
- increased lean body mass / MGI
- abnormal rod electrophysiology / MGI
- abnormal cone electrophysiology / MGI
- caudal body truncation / MGI
- abnormal vitelline vascular remodeling / MGI
- abnormal sarcomere morphology / MGI
- increased compact bone thickness / MGI
- transmission ratio distortion / MGI
- abnormal basement membrane morphology / MGI
- short frontal bone / MGI
- short nasal bone / MGI
- increased incidence of tumors by chemical induction / MGI
- caudal vertebral transformation / MGI
- cervical vertebral transformation / MGI
- lumbar vertebral transformation / MGI
- thoracic vertebral transformation / MGI
- cervical vertebral fusion / MGI
- absent caudal vertebrae / MGI
- small sacral vertebrae / MGI
- abnormal vertebral column morphology / MGI
- notochord degeneration / MGI
- truncated notochord / MGI
- abnormal platelet morphology / MGI
- decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
- increased hematopoietic stem cell number / MGI
- decreased testis weight / MGI
- increased energy expenditure / MGI
- decreased adiponectin level / MGI
- decreased epididymis weight / MGI
- abnormal epididymis epithelium morphology / MGI
- skin inflammation / MGI
- decreased B-1 B cell number / MGI
- increased neuronal precursor cell number / MGI
- increased osteoblast cell number / MGI
- decreased lymphocyte cell number / MGI
- decreased cardiac muscle contractility / MGI
- cachexia / MGI
- increased susceptibility to injury / MGI
- decreased circulating cholesterol level / MGI
- abnormal retinal pigment epithelium morphology / MGI
- abnormal rostral-caudal axis patterning / MGI
- glomerulosclerosis / MGI
- increased oxygen consumption / MGI
- impaired glucose tolerance / MGI
- spina bifida occulta / MGI
- decreased triglyceride level / MGI
- abnormal renal glomerulus morphology / MGI
- insulin resistance / MGI
- muscle phenotype / MGI
- homeostasis/metabolism phenotype / MGI
- growth/size/body region phenotype / MGI
- endocrine/exocrine gland phenotype / MGI
- cardiovascular system phenotype / MGI
- immune system phenotype / MGI
- reproductive system phenotype / MGI
- skeleton phenotype / MGI
- hematopoietic system phenotype / MGI
- increased circulating thyroxine level / MGI
- increased circulating triiodothyronine level / MGI
- abnormal skeleton morphology / MGI
- abnormal body temperature / MGI
- choroidal neovascularization / MGI
- abnormal eye electrophysiology / MGI
- decreased circulating glucose level / MGI
- increased mean corpuscular hemoglobin / MGI
- decreased mean corpuscular hemoglobin / MGI
- decreased angiogenesis / MGI
- increased bone mass / MGI
- abnormal cell physiology / MGI
- increased susceptibility to diet-induced obesity / MGI
- decreased susceptibility to diet-induced obesity / MGI
- decreased circulating leptin level / MGI
- increased circulating leptin level / MGI
- increased mitochondrial fission / MGI
- increased apoptosis / MGI
- abnormal tail development / MGI
- abnormal spermatid morphology / MGI
- intestine polyps / MGI
- abnormal paraxial mesoderm morphology / MGI
- abnormal podocyte slit diaphragm morphology / MGI
- decreased CD4-positive, alpha beta T cell number / MGI
- decreased CD8-positive, alpha-beta T cell number / MGI
- abnormal granulocyte differentiation / MGI
- abnormal monocyte differentiation / MGI
- podocyte foot process effacement / MGI
- increased germinal center B cell number / MGI
- decreased B-2 B cell number / MGI
- decreased mature B cell number / MGI
- increased megakaryocyte cell number / MGI
- abnormal bone ossification / MGI
- retinal photoreceptor degeneration / MGI
- abnormal medium spiny neuron morphology / MGI
- retinal outer nuclear layer degeneration / MGI
- increased circulating tumor necrosis factor level / MGI
- increased circulating interleukin-1 alpha level / MGI
- increased circulating interleukin-1 beta level / MGI
- increased interleukin-17 secretion / MGI
- decreased survivor rate / MGI
- increased B cell apoptosis / MGI
- increased liver iron level / MGI
- increased spleen iron level / MGI
- increased circulating iron level / MGI
- lipofuscinosis / MGI
- abnormal physiological response to xenobiotic / MGI
- decreased erythroid progenitor cell number / MGI
- anal stenosis / MGI
- hairpin sperm flagellum / MGI
- increased abdominal fat pad weight / MGI
- increased trophoblast giant cell number / MGI
- centrally nucleated skeletal muscle fibers / MGI
- skeletal muscle endomysial fibrosis / MGI
- abnormal synaptonemal complex / MGI
- abnormal mammary gland duct morphology / MGI
- decreased thymocyte apoptosis / MGI
- enlarged popliteal lymph nodes / MGI
- decreased birth body size / MGI
- impaired somite development / MGI
- increased total body fat amount / MGI
- abnormal placenta physiology / MGI
- abnormal retinal blood vessel morphology / MGI
- decreased mammary gland epithelial cell proliferation / MGI
- decreased tumor latency / MGI
- altered tumor pathology / MGI
- mortality/aging / MGI
- integument phenotype / MGI
- increased bone volume / MGI
- abnormal double-strand DNA break repair / MGI
- neonatal lethality, complete penetrance / MGI
- perinatal lethality, incomplete penetrance / MGI
- prenatal lethality, complete penetrance / MGI
- embryonic lethality during organogenesis, complete penetrance / MGI
- preweaning lethality, complete penetrance / MGI
- prenatal lethality, incomplete penetrance / MGI
- embryonic lethality during organogenesis, incomplete penetrance / MGI
- abnormal anterior visceral endoderm cell migration / MGI
- increased circulating erythropoietin level / MGI
- renal tubule atrophy / MGI
- lethality, complete penetrance / MGI
- increased mitochondria number / MGI
- rectal atresia / MGI
- rectourethral fistula / MGI
- increased hematopoietic stem cell proliferation / MGI
- increased food intake / MGI
- impaired mammary gland growth during pregnancy / MGI
- decreased paraxial mesoderm size / MGI
- abnormal skeletal muscle regeneration / MGI
- testis degeneration / MGI
- preputial gland inflammation / MGI
- nervous system inclusion bodies / MGI
- decreased fatty acid beta-oxidation / MGI
- epididymis hypertrophy / MGI
- spermatocele / MGI
- epididymis fibrosis / MGI
- increased epididymal cell proliferation / MGI
- increased epididymal epithelium cell proliferation / MGI
- increased heart iron level / MGI
- increased intestinal iron level / MGI
Literature references
- Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice.;Mort Richard Lester, Ford Matthew Jonathan, Sakaue-Sawano Asako, Lindstrom Nils Olof, Casadio Angela, Douglas Adam Thomas, Keighren Margaret Anne, Hohenstein Peter, Miyawaki Atsushi, Jackson Ian James, ;2014;Cell cycle (Georgetown, Tex.);13;2681-96; 25486356
- Endocycle-related tubular cell hypertrophy and progenitor proliferation recover renal function after acute kidney injury.;Lazzeri Elena, Angelotti Maria Lucia, Peired Anna, Conte Carolina, Marschner Julian A, Maggi Laura, Mazzinghi Benedetta, Lombardi Duccio, Melica Maria Elena, Nardi Sara, Ronconi Elisa, Sisti Alessandro, Antonelli Giulia, Becherucci Francesca, De Chiara Letizia, Guevara Ricardo Romero, Burger Alexa, Schaefer Beat, Annunziato Francesco, Anders Hans-Joachim, Lasagni Laura, Romagnani Paola, ;2018;Nature communications;9;1344; 29632300
- Tubular cell polyploidy protects from lethal acute kidney injury but promotes consequent chronic kidney disease.;De Chiara Letizia, Conte Carolina, Semeraro Roberto, Diaz-Bulnes Paula, Angelotti Maria Lucia, Mazzinghi Benedetta, Molli Alice, Antonelli Giulia, Landini Samuela, Melica Maria Elena, Peired Anna Julie, Maggi Laura, Donati Marta, La Regina Gilda, Allinovi Marco, Ravaglia Fiammetta, Guasti Daniele, Bani Daniele, Cirillo Luigi, Becherucci Francesca, Guzzi Francesco, Magi Alberto, Annunziato Francesco, Lasagni Laura, Anders Hans-Joachim, Lazzeri Elena, Romagnani Paola, ;2022;Nature communications;13;5805; 36195583
- Hair follicle dermal condensation forms via Fgf20 primed cell cycle exit, cell motility, and aggregation.;Biggs Leah C, Mäkelä Otto Jm, Myllymäki Satu-Marja, Das Roy Rishi, Närhi Katja, Pispa Johanna, Mustonen Tuija, Mikkola Marja L, ;2018;eLife;7;C849-C861; 30063206
- Polyploid tubular cells initiate a TGF-β1 controlled loop that sustains polyploidization and fibrosis after acute kidney injury.;De Chiara Letizia, Semeraro Roberto, Mazzinghi Benedetta, Landini Samuela, Molli Alice, Antonelli Giulia, Angelotti Maria Lucia, Melica Maria Elena, Maggi Laura, Conte Carolina, Peired Anna Julie, Cirillo Luigi, Raglianti Valentina, Magi Alberto, Annunziato Francesco, Romagnani Paola, Lazzeri Elena, ;2023;American journal of physiology. Cell physiology;325;4879; 37642236
- Protein translation rate determines neocortical neuron fate.;Borisova Ekaterina, Newman Andrew G, Couce Iglesias Marta, Dannenberg Rike, Schaub Theres, Qin Bo, Rusanova Alexandra, Brockmann Marisa, Koch Janina, Daniels Marieatou, Turko Paul, Jahn Olaf, Kaplan David R, Rosário Marta, Iwawaki Takao, Spahn Christian M T, Rosenmund Christian, Meierhofer David, Kraushar Matthew L, Tarabykin Victor, Ambrozkiewicz Mateusz C, ;2024;Nature communications;15;; 38849354
- In Vivo Imaging of Liver Spheroids Engrafted in the Anterior Chamber of the Mouse Eye.;Lazzeri-Barcelo Francesca, Ciardo Pierpaolo, Leibiger Barbara, Leibiger Ingo B, Berggren Per-Olof, Moruzzi Noah, ;2024;Journal of visualized experiments : JoVE;0;; 38619277