B6.129P2(Cg)-Tnfrsf1atm3.1Gkl/Flmg
| Status | Available to order |
| EMMA ID | EM:07099 |
| International strain name | B6.129P2(Cg)-Tnfrsf1atm3.1Gkl/Flmg |
| Alternative name | TNFRFL/FL or p55TNFRFL/FL, B6.129-Tnfrsf1a |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Tnfrsf1atm3.1Gkl, |
| Gene/Transgene symbol | Tnfrsf1a |
Information from provider
| Provider | George Kollias |
| Provider affiliation | Institute of Immunology, B.S.R.C. Al. Fleming |
| Genetic information | Exons 2-5 of Tnfrsf1a gene were flanked by loxP sites so to result in a frameshift mutation upon recombination. Upstream the first loxP site a flipped neo cassette has been inserted and flanking sequences of 3.6 and 2.8 kb 5prime and 3prime. Sequences of the mouse Tnfrsf1a gene were derived by PCR amplification from the respective BAC clone. Exons have been sequenced and the integrity of the sequences confirmed. The targeting vector was introduced in 129/Ola ES cells and positive clones used for the generation of chimaeras. Chimaeric mice have been crossed to C57BL/6J mice and germline offspring used for expansion. The mice have been crossed to FLP deleter mice for neo cassette removal. Then, Tnfrsf1a;flx/flx mice were crossed with Cre deleter mice for the generation of Tnfrsf1a complete ko. The Tnfrsf1a;flx/flx mouse line was expanded and the mice backcrossed to C57BL/6J for at least 6 generations. The deficiency in p55TNFR expression in the ko was confirmed in BMDMs by FACs analysis. |
| Phenotypic information | This is a strain that can be used with tissue specific Cre expressing strains to induce Cre-mediated TNFRI ablation. |
| Breeding history | Backcrossed in C57BL/6J, more than 10 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | B.S.R.C. Alexander Fleming, Vari, Greece |
| Animals used for archiving | homozygous 0, wild-type 0 |
| Stage of embryos | Morula |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Tumor necrosis factor receptor 1 associated periodic syndrome / Orphanet_32960
Literature references
- Safe TNF-based antitumor therapy following p55TNFR reduction in intestinal epithelium.;Van Hauwermeiren Filip, Armaka Marietta, Karagianni Niki, Kranidioti Ksanthi, Vandenbroucke Roosmarijn E, Loges Sonja, Van Roy Maarten, Staelens Jan, Puimège Leen, Palagani Ajay, Berghe Wim Vanden, Victoratos Panayiotis, Carmeliet Peter, Libert Claude, Kollias George, ;2013;The Journal of clinical investigation;123;2590-603; 23676465
- Tumor necrosis factor alpha receptor 1 deficiency in hepatocytes does not protect from non-alcoholic steatohepatitis, but attenuates insulin resistance in mice.;Bluemel Sena, Wang Yanhan, Lee Suhan, Schnabl Bernd, ;2020;World journal of gastroenterology;26;4933-4944; 32952340