B6.129P2-Gja10tm2(HBEGF/EGFP)Kwi/Cnrm

Status

Available to order

EMMA IDEM:06024
International strain nameB6.129P2-Gja10tm2(HBEGF/EGFP)Kwi/Cnrm
Alternative nameCx57-DTRfrtCre
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolGja10tm2(HBEGF/EGFP)Kwi,
Gene/Transgene symbolGja10

Information from provider

ProviderKlaus Willecke
Provider affiliationLIMES Institute, University of Bonn
Genetic informationThe primate diphtheria toxin receptor (DTR, or heparin-binding epidermal growth factor-like growth factor - HBEGF) is inserted into the mouse Gja10 (Cx57) locus leading to expression of the DTR in horizontal cells of the retina. Upon flp recombinase-mediated deletion, the DTR is exchanged for the cre recombinase.
Phenotypic informationAllows ablation of retinal horizontal cells by systemic application of diphtheria toxin; without toxin administration, mice show the same phenotype as described for Cx57lacZ (EM:01644).
Breeding historyThe line was backcrossed at least five times to C57BL/6N. As the C57BL/6N background can harbour the Crb1rd8 (crumbs family member 1, retinal degeneration 8) mutation, researchers who want to use this transgenic strain for studying retinal phenotypes should check for the rd8 locus. For future experiments backcrossing to C57BL/6J would be recommended. The primer combination proposed in the paper by Mattapallil et al., 2012 (IOVS, Vol 53, No. 6, 2921-2927) should be useful to eliminate the rd8 mutation.
References
  • Epigenetic regulation of promiscuous gene expression in thymic medullary epithelial cells.;Tykocinski Lars-Oliver, Sinemus Anna, Rezavandy Esmail, Weiland Yanina, Baddeley David, Cremer Christoph, Sonntag Stephan, Willecke Klaus, Derbinski Jens, Kyewski Bruno, ;2010;Proceedings of the National Academy of Sciences of the United States of America;107;19426-31; 20966351
  • Ablation of retinal horizontal cells from adult mice leads to rod degeneration and remodeling in the outer retina.;Sonntag Stephan, Dedek Karin, Dorgau Birthe, Schultz Konrad, Schmidt Karl-Friedrich, Cimiotti Kerstin, Weiler Reto, Löwel Siegrid, Willecke Klaus, Janssen-Bienhold Ulrike, ;2012;The Journal of neuroscience : the official journal of the Society for Neuroscience;32;10713-24; 22855819
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

MGI phenotypes (allele matching)
  • abnormal retinal rod cell morphology / MGI
  • abnormal retinal cone cell morphology / MGI
  • abnormal retina morphology / MGI
  • abnormal vision / MGI
  • abnormal eye electrophysiology / MGI
  • retinal rod cell degeneration / MGI
  • thin retinal outer plexiform layer / MGI
  • disorganized retinal outer plexiform layer / MGI

Literature references

  • Epigenetic regulation of promiscuous gene expression in thymic medullary epithelial cells.;Tykocinski Lars-Oliver, Sinemus Anna, Rezavandy Esmail, Weiland Yanina, Baddeley David, Cremer Christoph, Sonntag Stephan, Willecke Klaus, Derbinski Jens, Kyewski Bruno, ;2010;Proceedings of the National Academy of Sciences of the United States of America;107;19426-31; 20966351
  • Ablation of retinal horizontal cells from adult mice leads to rod degeneration and remodeling in the outer retina.;Sonntag Stephan, Dedek Karin, Dorgau Birthe, Schultz Konrad, Schmidt Karl-Friedrich, Cimiotti Kerstin, Weiler Reto, Löwel Siegrid, Willecke Klaus, Janssen-Bienhold Ulrike, ;2012;The Journal of neuroscience : the official journal of the Society for Neuroscience;32;10713-24; 22855819

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
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Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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