B6.Cg-Tg(Met,-lacZ)#Buck/Orl
| Status | Available to order |
| EMMA ID | EM:05742 |
| Citation information | RRID:IMSR_EM:05742 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Tg(Met,-lacZ)#Buck/Orl |
| Alternative name | B6.P34TKZI |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Pax3CSS)5-TK-nLacZ |
| Gene/Transgene symbol | Tg(Pax3CSS)5-TK-nLacZ |
Information from provider
| Provider | Frederic RELAIX |
| Provider affiliation | Groupe Myologie, UMR-S 787 - INSERM - UPMC-Paris VI - Institut de Myologie |
| Additional owner | Margaret Buckingham, Molecular Genetics of Development (URA CNRS 2578), Institut Pasteur, Paris, France |
| Genetic information | Oligonucleotides containing the Pax3 consensus binding site defined by Epstein et al. (1996) were multimerized. The sequence of the oligonucleotides is as follows: CSSPax3-A, 5-CCTCGTCACGCTTCGAATGT-3; and CSSPax3-B, 5-GGGACATTCGAAGCGTGACGA-3. The oligonucleotides were annealed and polymerized. Concatenamers containing five binding sites were gel-purified, and cloned into a TK-nLacZpA vector, which contains the nlacZ reporter gene under the control of the thymidine kinase (TK) promoter (Hadchouel et al. 2003), into an SfiI site located 5 to the TK minimal promoter. A 7.4-kb plasmid fragment for injection was isolated using XhoI and NotI as previously described (Kelly et al. 1995). Transgenic mice were generated as previously described (Hadchouel et al. 2003). |
| Phenotypic information | Normal, lacZ reporter for Pax3 gene |
| Breeding history | The line has been backcrossed to C57BL/6 background for at least 10 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6J males, wild-type C57BL/6J females |
| Stage of embryos | 2-cell |
Literature references
- Divergent functions of murine Pax3 and Pax7 in limb muscle development.;Relaix Frédéric, Rocancourt Didier, Mansouri Ahmed, Buckingham Margaret, ;2004;Genes & development;18;1088-105; 15132998
- The transcriptional activator PAX3-FKHR rescues the defects of Pax3 mutant mice but induces a myogenic gain-of-function phenotype with ligand-independent activation of Met signaling in vivo.;Relaix Frédéric, Polimeni Mariarosa, Rocancourt Didier, Ponzetto Carola, Schäfer Beat W, Buckingham Margaret, ;2003;Genes & development;17;2950-65; 14665670