B6.Cg-Tg(Met,-lacZ)#Buck/Orl
| Status | Available to order |
| EMMA ID | EM:05742 |
| International strain name | B6.Cg-Tg(Met,-lacZ)#Buck/Orl |
| Alternative name | B6.P34TKZI |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Pax3CSS)5-TK-nLacZ |
| Gene/Transgene symbol | Tg(Pax3CSS)5-TK-nLacZ |
Information from provider
| Provider | Frederic RELAIX |
| Provider affiliation | Groupe Myologie, UMR-S 787 - INSERM - UPMC-Paris VI - Institut de Myologie |
| Additional owner | Margaret Buckingham, Molecular Genetics of Development (URA CNRS 2578), Institut Pasteur, Paris, France |
| Genetic information | Oligonucleotides containing the Pax3 consensus binding site defined by Epstein et al. (1996) were multimerized. The sequence of the oligonucleotides is as follows: CSSPax3-A, 5-CCTCGTCACGCTTCGAATGT-3; and CSSPax3-B, 5-GGGACATTCGAAGCGTGACGA-3. The oligonucleotides were annealed and polymerized. Concatenamers containing five binding sites were gel-purified, and cloned into a TK-nLacZpA vector, which contains the nlacZ reporter gene under the control of the thymidine kinase (TK) promoter (Hadchouel et al. 2003), into an SfiI site located 5 to the TK minimal promoter. A 7.4-kb plasmid fragment for injection was isolated using XhoI and NotI as previously described (Kelly et al. 1995). Transgenic mice were generated as previously described (Hadchouel et al. 2003). |
| Phenotypic information | Normal, lacZ reporter for Pax3 gene |
| Breeding history | The line has been backcrossed to C57BL/6 background for at least 10 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6J, wild-type C57BL/6J |
| Stage of embryos | 2-cell |
Literature references
- Divergent functions of murine Pax3 and Pax7 in limb muscle development.;Relaix Frédéric, Rocancourt Didier, Mansouri Ahmed, Buckingham Margaret, ;2004;Genes & development;18;1088-105; 15132998
- The transcriptional activator PAX3-FKHR rescues the defects of Pax3 mutant mice but induces a myogenic gain-of-function phenotype with ligand-independent activation of Met signaling in vivo.;Relaix Frédéric, Polimeni Mariarosa, Rocancourt Didier, Ponzetto Carola, Schäfer Beat W, Buckingham Margaret, ;2003;Genes & development;17;2950-65; 14665670