B6.129S-Tnfrsf1atm2Gkl/Flmg
| Status | Available to order |
| EMMA ID | EM:04792 |
| International strain name | B6.129S-Tnfrsf1atm2Gkl/Flmg |
| Alternative name | TNFRI |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Tnfrsf1atm2Gkl, |
| Gene/Transgene symbol | Tnfrsf1a |
Information from provider
| Provider | George Kollias |
| Provider affiliation | B.S.R.C. |
| Genetic information | A targeting vector containing a 4.6 kb XbaI/SmaI genomic fragment as the left homology arm, a 4 kb SmaI/HindIII genomic fragment as the right homology arm, a loxP-flanked neo selection cassette (L2neo) introduced into a SmaI site in intron 5 of the p55TNFR (Tnfrsf1a) gene between the two homology arms and a PGK-tk negative selection marker at the 3' of the targeting vector, was transfected into ES cells for the generation of the p55cneo allele. |
| Phenotypic information | Mutant mice carry a conditional gain-of-function allele (p55TnfRcneo) obtained by introducing a loxP-flanked neomycin resistance cassette in intron 5 of the murine p55TnfR (Tnfrsf1a) gene. p55TNFR expression is inhibited by the presence of the neomycin cassette but it is restored upon Cre-mediated neo excision. |
| Breeding history | Backcrossed for more than 12 generations to C57BL/6J. |
| References |
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Information from EMMA
| Archiving centre | B.S.R.C. Alexander Fleming, Vari, Greece |
| Animals used for archiving | homozygous C57BL/6, wild-type C57BL/6 |
| Stage of embryos | 2-cell |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Tumor necrosis factor receptor 1 associated periodic syndrome / Orphanet_32960
Literature references
- FDC-specific functions of p55TNFR and IKK2 in the development of FDC networks and of antibody responses.;Victoratos Panayiotis, Lagnel Jacques, Tzima Sotiria, Alimzhanov Marat B, Rajewsky Klaus, Pasparakis Manolis, Kollias George, ;2006;Immunity;24;65-77; 16413924