129S2-Prkaa1tm1Sbj/Orl

Status

Available to order

EMMA IDEM:02139
International strain name129S2-Prkaa1tm1Sbj/Orl
Alternative nameAMPKalpha1 knockout
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolPrkaa1tm1Sbj
Gene/Transgene symbolPrkaa1

Information from provider

ProviderBenoit Viollet
Provider affiliationINSERMU567, CNRS UMR8401, universite Paris 5, Institut Cochin
Genetic informationSequence encoding amino acids 97 through 157, which make up a portion of the catalytic domain, was replaced with an IRES-betageo cassette inserted by homologous recombination.
Phenotypic informationAnalysis in progress. No defect in glucose homoeostasis was observed in AMPKalpha1 (Prkaa1) -/- mice.
References
  • Knockout of the alpha2 but not alpha1 5'-AMP-activated protein kinase isoform abolishes 5-aminoimidazole-4-carboxamide-1-beta-4-ribofuranosidebut not contraction-induced glucose uptake in skeletal muscle.;Jørgensen Sebastian B, Viollet Benoit, Andreelli Fabrizio, Frøsig Christian, Birk Jesper B, Schjerling Peter, Vaulont Sophie, Richter Erik A, Wojtaszewski Jørgen F P, ;2004;The Journal of biological chemistry;279;1070-9; 14573616
  • Anti-lipolytic action of AMP-activated protein kinase in rodent adipocytes.;Daval Marie, Diot-Dupuy Francine, Bazin Raymond, Hainault Isabelle, Viollet Benoît, Vaulont Sophie, Hajduch Eric, Ferré Pascal, Foufelle Fabienne, ;2005;The Journal of biological chemistry;280;25250-7; 15878856
  • Effects of alpha-AMPK knockout on exercise-induced gene activation in mouse skeletal muscle.;Jørgensen Sebastian B, Wojtaszewski Jørgen F P, Viollet Benoit, Andreelli Fabrizio, Birk Jesper B, Hellsten Ylva, Schjerling Peter, Vaulont Sophie, Neufer P Darrell, Richter Erik A, Pilegaard Henriette, ;2005;FASEB journal : official publication of the Federation of American Societies for Experimental Biology;19;1146-8; 15878932
  • Understanding the molecular basis of the interaction between NDPK-A and AMPK alpha 1.;Crawford Russell M, Treharne Kate J, Arnaud-Dabernat Sandrine, Daniel Jean-Yves, Foretz Marc, Viollet Benoit, Mehta Anil, ;2006;Molecular and cellular biology;26;5921-31; 16847342
  • Polyunsaturated fatty acids suppress glycolytic and lipogenic genes through the inhibition of ChREBP nuclear protein translocation.;Dentin Renaud, Benhamed Fadila, Pégorier Jean-Paul, Foufelle Fabienne, Viollet Benoit, Vaulont Sophie, Girard Jean, Postic Catherine, ;2005;The Journal of clinical investigation;115;2843-54; 16184193
  • Activation of the AMP-activated kinase by antidiabetes drug metformin stimulates nitric oxide synthesis in vivo by promoting the association of heat shock protein 90 and endothelial nitric oxide synthase.;Davis Bradley J, Xie Zhonglin, Viollet Benoit, Zou Ming-Hui, ;2006;Diabetes;55;496-505; 16443786

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France

Disease and phenotype information

MGI phenotypes (allele matching)
  • decreased muscle cell glucose uptake / MGI
  • decreased hematocrit / MGI
  • hemolytic anemia / MGI
  • low mean erythrocyte cell number / MGI
  • reticulocytosis / MGI
  • abnormal erythrocyte osmotic lysis / MGI
  • increased spleen weight / MGI
  • abnormal circulating hormone level / MGI
  • decreased mean corpuscular hemoglobin / MGI
  • increased red blood cell distribution width / MGI
MGI phenotypes (gene matching)
  • decreased hematocrit / MGI
  • hemolytic anemia / MGI
  • low mean erythrocyte cell number / MGI
  • reticulocytosis / MGI
  • abnormal erythrocyte osmotic lysis / MGI
  • increased spleen weight / MGI
  • abnormal circulating hormone level / MGI
  • decreased mean corpuscular hemoglobin / MGI
  • increased red blood cell distribution width / MGI
  • decreased muscle cell glucose uptake / MGI

Literature references

  • Knockout of the alpha2 but not alpha1 5'-AMP-activated protein kinase isoform abolishes 5-aminoimidazole-4-carboxamide-1-beta-4-ribofuranosidebut not contraction-induced glucose uptake in skeletal muscle.;Jørgensen Sebastian B, Viollet Benoit, Andreelli Fabrizio, Frøsig Christian, Birk Jesper B, Schjerling Peter, Vaulont Sophie, Richter Erik A, Wojtaszewski Jørgen F P, ;2004;The Journal of biological chemistry;279;1070-9; 14573616
  • Anti-lipolytic action of AMP-activated protein kinase in rodent adipocytes.;Daval Marie, Diot-Dupuy Francine, Bazin Raymond, Hainault Isabelle, Viollet Benoît, Vaulont Sophie, Hajduch Eric, Ferré Pascal, Foufelle Fabienne, ;2005;The Journal of biological chemistry;280;25250-7; 15878856
  • Effects of alpha-AMPK knockout on exercise-induced gene activation in mouse skeletal muscle.;Jørgensen Sebastian B, Wojtaszewski Jørgen F P, Viollet Benoit, Andreelli Fabrizio, Birk Jesper B, Hellsten Ylva, Schjerling Peter, Vaulont Sophie, Neufer P Darrell, Richter Erik A, Pilegaard Henriette, ;2005;FASEB journal : official publication of the Federation of American Societies for Experimental Biology;19;1146-8; 15878932
  • Understanding the molecular basis of the interaction between NDPK-A and AMPK alpha 1.;Crawford Russell M, Treharne Kate J, Arnaud-Dabernat Sandrine, Daniel Jean-Yves, Foretz Marc, Viollet Benoit, Mehta Anil, ;2006;Molecular and cellular biology;26;5921-31; 16847342
  • Polyunsaturated fatty acids suppress glycolytic and lipogenic genes through the inhibition of ChREBP nuclear protein translocation.;Dentin Renaud, Benhamed Fadila, Pégorier Jean-Paul, Foufelle Fabienne, Viollet Benoit, Vaulont Sophie, Girard Jean, Postic Catherine, ;2005;The Journal of clinical investigation;115;2843-54; 16184193
  • Activation of the AMP-activated kinase by antidiabetes drug metformin stimulates nitric oxide synthesis in vivo by promoting the association of heat shock protein 90 and endothelial nitric oxide synthase.;Davis Bradley J, Xie Zhonglin, Viollet Benoit, Zou Ming-Hui, ;2006;Diabetes;55;496-505; 16443786

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