129S2-Prkaa1^tm1Sbj/Orl

Status	Available to order
EMMA ID	EM:02139
Citation information	RRID:IMSR_EM:02139 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	129S2-Prkaa1^tm1Sbj/Orl
Alternative name	AMPKalpha1 knockout
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Prkaa1^tm1Sbj
Gene/Transgene symbol	Prkaa1

Information from provider

Provider	Benoit Viollet
Provider affiliation	INSERMU567, CNRS UMR8401, universite Paris 5, Institut Cochin
Genetic information	Sequence encoding amino acids 97 through 157, which make up a portion of the catalytic domain, was replaced with an IRES-betageo cassette inserted by homologous recombination.
Phenotypic information	Analysis in progress. No defect in glucose homoeostasis was observed in AMPKalpha1 (Prkaa1) -/- mice.
References	Knockout of the alpha2 but not alpha1 5'-AMP-activated protein kinase isoform abolishes 5-aminoimidazole-4-carboxamide-1-beta-4-ribofuranosidebut not contraction-induced glucose uptake in skeletal muscle.;Jørgensen Sebastian B, Viollet Benoit, Andreelli Fabrizio, Frøsig Christian, Birk Jesper B, Schjerling Peter, Vaulont Sophie, Richter Erik A, Wojtaszewski Jørgen F P, ;2004;The Journal of biological chemistry;279;1070-9; 14573616 Anti-lipolytic action of AMP-activated protein kinase in rodent adipocytes.;Daval Marie, Diot-Dupuy Francine, Bazin Raymond, Hainault Isabelle, Viollet Benoît, Vaulont Sophie, Hajduch Eric, Ferré Pascal, Foufelle Fabienne, ;2005;The Journal of biological chemistry;280;25250-7; 15878856 Effects of alpha-AMPK knockout on exercise-induced gene activation in mouse skeletal muscle.;Jørgensen Sebastian B, Wojtaszewski Jørgen F P, Viollet Benoit, Andreelli Fabrizio, Birk Jesper B, Hellsten Ylva, Schjerling Peter, Vaulont Sophie, Neufer P Darrell, Richter Erik A, Pilegaard Henriette, ;2005;FASEB journal : official publication of the Federation of American Societies for Experimental Biology;19;1146-8; 15878932 Understanding the molecular basis of the interaction between NDPK-A and AMPK alpha 1.;Crawford Russell M, Treharne Kate J, Arnaud-Dabernat Sandrine, Daniel Jean-Yves, Foretz Marc, Viollet Benoit, Mehta Anil, ;2006;Molecular and cellular biology;26;5921-31; 16847342 Polyunsaturated fatty acids suppress glycolytic and lipogenic genes through the inhibition of ChREBP nuclear protein translocation.;Dentin Renaud, Benhamed Fadila, Pégorier Jean-Paul, Foufelle Fabienne, Viollet Benoit, Vaulont Sophie, Girard Jean, Postic Catherine, ;2005;The Journal of clinical investigation;115;2843-54; 16184193 Activation of the AMP-activated kinase by antidiabetes drug metformin stimulates nitric oxide synthesis in vivo by promoting the association of heat shock protein 90 and endothelial nitric oxide synthase.;Davis Bradley J, Xie Zhonglin, Viollet Benoit, Zou Ming-Hui, ;2006;Diabetes;55;496-505; 16443786

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France

Disease and phenotype information

MGI phenotypes (allele matching)

decreased muscle cell glucose uptake / MGI
decreased hematocrit / MGI
hemolytic anemia / MGI
low mean erythrocyte cell number / MGI
reticulocytosis / MGI
abnormal erythrocyte osmotic lysis / MGI
increased spleen weight / MGI
abnormal circulating hormone level / MGI
decreased mean corpuscular hemoglobin / MGI
increased red blood cell distribution width / MGI

MGI phenotypes (gene matching)

decreased hematocrit / MGI
hemolytic anemia / MGI
low mean erythrocyte cell number / MGI
reticulocytosis / MGI
abnormal erythrocyte osmotic lysis / MGI
increased spleen weight / MGI
abnormal circulating hormone level / MGI
decreased mean corpuscular hemoglobin / MGI
increased red blood cell distribution width / MGI
decreased muscle cell glucose uptake / MGI

Literature references

Knockout of the alpha2 but not alpha1 5'-AMP-activated protein kinase isoform abolishes 5-aminoimidazole-4-carboxamide-1-beta-4-ribofuranosidebut not contraction-induced glucose uptake in skeletal muscle.;Jørgensen Sebastian B, Viollet Benoit, Andreelli Fabrizio, Frøsig Christian, Birk Jesper B, Schjerling Peter, Vaulont Sophie, Richter Erik A, Wojtaszewski Jørgen F P, ;2004;The Journal of biological chemistry;279;1070-9; 14573616
Anti-lipolytic action of AMP-activated protein kinase in rodent adipocytes.;Daval Marie, Diot-Dupuy Francine, Bazin Raymond, Hainault Isabelle, Viollet Benoît, Vaulont Sophie, Hajduch Eric, Ferré Pascal, Foufelle Fabienne, ;2005;The Journal of biological chemistry;280;25250-7; 15878856
Effects of alpha-AMPK knockout on exercise-induced gene activation in mouse skeletal muscle.;Jørgensen Sebastian B, Wojtaszewski Jørgen F P, Viollet Benoit, Andreelli Fabrizio, Birk Jesper B, Hellsten Ylva, Schjerling Peter, Vaulont Sophie, Neufer P Darrell, Richter Erik A, Pilegaard Henriette, ;2005;FASEB journal : official publication of the Federation of American Societies for Experimental Biology;19;1146-8; 15878932
Understanding the molecular basis of the interaction between NDPK-A and AMPK alpha 1.;Crawford Russell M, Treharne Kate J, Arnaud-Dabernat Sandrine, Daniel Jean-Yves, Foretz Marc, Viollet Benoit, Mehta Anil, ;2006;Molecular and cellular biology;26;5921-31; 16847342
Polyunsaturated fatty acids suppress glycolytic and lipogenic genes through the inhibition of ChREBP nuclear protein translocation.;Dentin Renaud, Benhamed Fadila, Pégorier Jean-Paul, Foufelle Fabienne, Viollet Benoit, Vaulont Sophie, Girard Jean, Postic Catherine, ;2005;The Journal of clinical investigation;115;2843-54; 16184193
Activation of the AMP-activated kinase by antidiabetes drug metformin stimulates nitric oxide synthesis in vivo by promoting the association of heat shock protein 90 and endothelial nitric oxide synthase.;Davis Bradley J, Xie Zhonglin, Viollet Benoit, Zou Ming-Hui, ;2006;Diabetes;55;496-505; 16443786

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Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

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Material Transfer Agreement (MTA)
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129S2-Prkaa1tm1Sbj/Orl