B6.129P2(C)-Igf2tm4.1Wrk/H

Status

Available to order

EMMA IDEM:01733
Citation informationRRID:IMSR_EM:01733 

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International strain nameB6.129P2(C)-Igf2tm4.1Wrk/H
Alternative nameS1.2Del
Strain typeTargeted Mutant Strains : Other targeted
Allele/Transgene symbolIgf2tm4.1Wrk
Gene/Transgene symbolIgf2

Information from provider

ProviderDr Wolf Reik
Provider affiliationThe Babraham Institute, Babraham, Cambridge, CB2 4PB
Genetic informationReplacement vector causing a small knock-out in a silencer region of the mouse Igf2 gene, in a repressor protein binding site, and leading to relative derepression of Igf2. In wild-type mice the Igf2 gene is imprinted on the maternal allele. The question addressed by this knock-out was to determine how much of the repression of the Igf2 maternal allele might be due to the presence of the upstream GCF2 (GC-binding factor 2, a known repression protein) binding site element located at HpaII site 4, in the differentially methylated region 1 (DMR1) of the Igf2 locus. The S1.2 DEL strain carries therefore a disruption of the GCF2 element caused by the insertion of a single loxP sequence within the GCF2 element. The GCF2 binding site mutation results in de-repression of the normally silent maternal allele (~3-fold more mRNA is produced compared with the wild-type maternal allele; see Eden et al. 2001, EMBO J, 20: 3518-3525 for more details). Note: the S1.2DEL strain was obtained by crossing the S1.2 Neo strain (EMMA strain EM:01732) with an ubiquitous expressor of the cre recombinase protein.
Phenotypic informationLoss of Igf2 imprinting.
References
  • An upstream repressor element plays a role in Igf2 imprinting.;Eden S, Constancia M, Hashimshony T, Dean W, Goldstein B, Johnson A C, Keshet I, Reik W, Cedar H, ;2001;The EMBO journal;20;3518-25; 11432838

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

MGI phenotypes (allele matching)
  • abnormal imprinting / MGI
  • growth/size/body region phenotype / MGI

Literature references

  • An upstream repressor element plays a role in Igf2 imprinting.;Eden S, Constancia M, Hashimshony T, Dean W, Goldstein B, Johnson A C, Keshet I, Reik W, Cedar H, ;2001;The EMBO journal;20;3518-25; 11432838

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Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

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