B6.Cg-Gt(ROSA)26Sortm1(Trib1,-EGFP) Lyz2tm1(cre)Ifo/H

Status

Available to order

EMMA IDEM:14886
International strain nameB6.Cg-Gt(ROSA)26Sortm1(Trib1,-EGFP) Lyz2tm1(cre)Ifo/H
Alternative nameTrib1 Rosa TG
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolLyz2tm1(cre)Ifo,
Gene/Transgene symbolLyz2

Information from provider

ProviderEndre Kiss-Toth
Provider affiliationIICD, University of Sheffield
Genetic informationMurine Trib1 complementary DNA was introduced into the previously described pROSA26, loxP-flanked STOP and Frt-flanked internal ribosomal entry site–eGFP targeting construct and then inserted into the ubiquitously expressed Rosa26 locus of Bruce4 (B6.Cg-Thy1a origin) mouse ES cells by homologous recombination; correct integration was confirmed by Southern blotting. Cre-mediated excision of the STOP cassette produces a bicistronic Trib1-eGFP transcript. The presently submitted strain results from further crossing to a Lyz2-cre recombinase-deleter strain, and it still carries the Lyz2-cre deleter construct, so that the re-animated line can be effectively used for het-het breeding to generate experimental and control littermates.
Phenotypic informationHomozygous:
No overt phenotype.

Heterozygous:
No overt phenotype.
References
  • Myeloid Tribbles 1 induces early atherosclerosis via enhanced foam cell expansion.;Johnston Jessica M, Angyal Adrienn, Bauer Robert C, Hamby Stephen, Suvarna S Kim, Baidžajevas Kajus, Hegedus Zoltan, Dear T Neil, Turner Martin, null null, Wilson Heather L, Goodall Alison H, Rader Daniel J, Shoulders Carol C, Francis Sheila E, Kiss-Toth Endre, ;2019;Science advances;5;eaax9183; 31692955
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

MGI phenotypes (allele matching)
  • enlarged spleen / MGI

Literature references

  • Myeloid Tribbles 1 induces early atherosclerosis via enhanced foam cell expansion.;Johnston Jessica M, Angyal Adrienn, Bauer Robert C, Hamby Stephen, Suvarna S Kim, Baidžajevas Kajus, Hegedus Zoltan, Dear T Neil, Turner Martin, null null, Wilson Heather L, Goodall Alison H, Rader Daniel J, Shoulders Carol C, Francis Sheila E, Kiss-Toth Endre, ;2019;Science advances;5;eaax9183; 31692955

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Practical information

Example health report
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Material Transfer Agreement (MTA)
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