B6NCrl.Cg-Uqcrhtm1b(EUCOMM)Wtsi Gt(ROSA)26Sortm1.1(CAG-AOX)Htj/Ieg

Status

Available to order

EMMA IDEM:13642
International strain nameB6NCrl.Cg-Uqcrhtm1b(EUCOMM)Wtsi Gt(ROSA)26Sortm1.1(CAG-AOX)Htj/Ieg
Alternative nameUqox_het
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolGt(ROSA)26Sortm1.1(CAG-AOX)Htj,
Gene/Transgene symbolGt(ROSA)26Sor

Information from provider

ProviderValerie Gailus-Durner
Provider affiliationGMC, IEG
Additional ownerUqox belongs to Dr. Valerie Gailus Durner; AOXrosa26 belongs to Dr. Marten Szibor, Jena University Hospital, Germany; Uqcrh belongs to Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Germany.
Genetic informationUqcrh_EPD0378_3_C07_tm1b-het + Gt(ROSA)26Sor_AOX_KI-het. Uqcrh-mutant mice were generated on a C57BL/6NCrl genetic background and maintained by het x wild-type maiting over >5 generations. Gt(ROSA)26Sor_AOX_KI (a.k.a. AOX-Rosa26-tg; MGI:5824337 allele ID) mice were originally generated and maintained on a C57BL/6J genetic background. Heterozygous animals were outcrossed to C57BL/6NCrl wild-type animals over seven generations, before the parental generation for the production of Uqox animals was generated (see below). Uqox mice were produced by mating in a first step C57BL/6NCrl Gt(ROSA)26Sor_AOX_KI heterozygous mice (AOX het) to C57BL/6NCrl Uqcrh-tm1b (MGI:5692661 allele ID; EMMA strain ID EM:10141) heterozygous animals (Uqcrh het) to produce double heterozygous animals. For preservation of the line sperm of Uqcrh het/AOX het males was used. In a second step Uqcrh het/AOX het offspring is mated to Uqcrh het/AOX wild-type offspring to produce Uqcrh hom/AOX het animals (so-called Uqox mice).
Phenotypic informationHomozygous:
Uqcrh_hom: low body weight; small; severe hyperglycemia from 6 weeks of age; reduced livespan (max. 11 weeks).

Heterozygous:
Uqcrh_het: none
Breeding historyGt(ROSA)26Sor_AOX_KI-het x Uqcrh_EPD0378_3_C07_tm1b-het.
References
  • AOX delays the onset of the lethal phenotype in a mouse model of Uqcrh (complex III) disease;Jacobs HT, Szibor M, Rathkolb B, da Silva-Buttkus P, Aguilar-Pimentel JA, Amarie OV, Becker L, Calzada-Wack J, Dragano N, Garrett L, Gerlini R, Hölter SM, Klein-Rodewald T, Kraiger M, Leuchtenberger S, Marschall S, Östereicher MA, Pfannes K, Sanz-Moreno A, Seisenberger C, Spielmann N, Stoeger C, Wurst W, Fuchs H, Hrabe de Angelis M, Gailus-Durner V.;2023;Biochim Biophys Acta Mol Basis Dis;1869;166760; 37230398
Homozygous fertilenot known
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisednot known

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivingheterozygous C57BL/6N

Literature references

  • AOX delays the onset of the lethal phenotype in a mouse model of Uqcrh (complex III) disease;Jacobs HT, Szibor M, Rathkolb B, da Silva-Buttkus P, Aguilar-Pimentel JA, Amarie OV, Becker L, Calzada-Wack J, Dragano N, Garrett L, Gerlini R, Hölter SM, Klein-Rodewald T, Kraiger M, Leuchtenberger S, Marschall S, Östereicher MA, Pfannes K, Sanz-Moreno A, Seisenberger C, Spielmann N, Stoeger C, Wurst W, Fuchs H, Hrabe de Angelis M, Gailus-Durner V.;2023;Biochim Biophys Acta Mol Basis Dis;1869;166760; 37230398

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
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Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

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Legally binding conditions for the transfer

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