C57BL/6-Slc20a2tm1c(EUCOMM)Wtsi/Orl

Status

Available to order

EMMA IDEM:13085
International strain nameC57BL/6-Slc20a2tm1c(EUCOMM)Wtsi/Orl
Alternative nameSlc20a2-tm1Nhch
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolSlc20a2tm1c(EUCOMM)Wtsi
Gene/Transgene symbolSlc20a2

Information from provider

ProviderNati Hernando
Provider affiliationPhysiology, University Zurich
Additional ownerDr. Carsten Wagner, Institute of Physiology, University Zurich, CH
Genetic informationEmbryonic stem (ES) cells transfected with a Slc20a2 targeting vector were purchased from the International Knockout Mouse Consortium (IKMC)/ European Conditional Mouse Mutagenesis Program (EUCOMM). In these ES cells, the exon 4 of the sodium/phosphate cotransporter Slc20a2/Pit-2 was flanked by loxP sites. Upstream of the 5’ loxP site, the vector contained a cassette for production of beta-galactosidase, followed by a neomycin phosphotransferase gene cassette. ES cells were sent to the Institute of Labortierkunde (LTK) of the University of Zurich for morula aggregation and implantation into foster mothers. Upon breeding to homozygosity, floxed/floxed mice were crossed with flp recombinase full deleters (64FlpB6) for removal of the beta-gal/neo cassettes.
Phenotypic informationHomozygous:
An intestinal-specific knockout was generated by breeding floxed Slc20a2 tm1c mice with villin-cre mice, in which the expression of the cre recombinase is under the control of the villin promoter. No differences in phosphate homeostasis were found between wild-types and homozygous littermates (Pastor-Arroyo et al., 2020, PubMed ID: 32564464).

Heterozygous:
Heterozygous mice were not analysed, but they are not expected to have any phenotype since homozygous mice did not show any.
Breeding historyMice breed without problems.
References
  • Intestinal epithelial ablation of Pit-2/Slc20a2 in mice leads to sustained elevation of vitamin D3 upon dietary restriction of phosphate.;Pastor-Arroyo Eva M, Knöpfel Thomas, Imenez Silva Pedro H, Schnitzbauer Udo, Poncet Nadège, Biber Jürg, Wagner Carsten A, Hernando Nati, ;2020;Acta physiologica (Oxford, England);230;e13526; 32564464
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredyes
Immunocompromisedno

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France

Disease and phenotype information

IMPC phenotypes (gene matching)
  • eyelids fail to open / IMPC
  • decreased total retina thickness / IMPC
  • cataract / IMPC
  • abnormal incisor color / IMPC
  • abnormal lens morphology / IMPC
  • decreased bone mineral density / IMPC
  • decreased bone mineral content / IMPC
  • impaired glucose tolerance / IMPC
  • abnormal vitreous body morphology / IMPC
  • increased circulating magnesium level / IMPC
  • developmental and structural abnormality / IMPC
  • increased total body fat amount / IMPC
  • developmental dysplasia / IMPC
  • decreased body length / IMPC
  • narrow eye opening / IMPC
  • preweaning lethality, incomplete penetrance / IMPC
  • abnormal vocalization / IMPC
  • abnormal spine curvature / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • abnormal retina vasculature morphology / IMPC
  • mineralisation / IMPC
  • irregularly shaped pupil / IMPC
  • increased grip strength / IMPC
  • kyphosis / IMPC
  • absent pinna reflex / IMPC
  • abnormal retina morphology / IMPC
  • abnormal auditory brainstem response / IMPC
  • abnormal iris morphology / IMPC
  • abnormal motor coordination/balance / IMPC
MGI phenotypes (gene matching)
  • abnormal mineral level / MGI
  • decreased circulating phosphate level / MGI
  • abnormal atrioventricular cushion morphology / MGI
  • abnormal forebrain morphology / MGI
  • abnormal cerebral cortex morphology / MGI
  • abnormal thalamus morphology / MGI
  • small superior cervical ganglion / MGI
  • ectopic cartilage / MGI
  • fusion of vertebral arches / MGI
  • abnormal basal ganglion morphology / MGI
  • abnormal vena cava morphology / MGI
  • arteriovenous malformation / MGI
  • microgliosis / MGI
  • abnormal liver vasculature morphology / MGI
  • anomalous pulmonary venous connection / MGI
  • abnormal hypoglossal nerve topology / MGI
  • subcutaneous edema / MGI
  • multiple persisting craniopharyngeal ducts / MGI
  • embryo tumor / MGI
  • embryo cyst / MGI
  • hemangioma / MGI
  • calcified brain / MGI

Literature references

  • Intestinal epithelial ablation of Pit-2/Slc20a2 in mice leads to sustained elevation of vitamin D3 upon dietary restriction of phosphate.;Pastor-Arroyo Eva M, Knöpfel Thomas, Imenez Silva Pedro H, Schnitzbauer Udo, Poncet Nadège, Biber Jürg, Wagner Carsten A, Hernando Nati, ;2020;Acta physiologica (Oxford, England);230;e13526; 32564464

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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