STOCK Tg(Prm1-EGFP)#Ltku/H
| Status | Available to order |
| EMMA ID | EM:12662 |
| International strain name | STOCK Tg(Prm1-EGFP)#Ltku/H |
| Alternative name | CD1; B6D2-Tg(Prm1EGFP, CAG-mRFP1, HS4i-TyrC)85Ltku |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Prm1-EGFP)#Ltku |
| Gene/Transgene symbol | Tg(Prm1-EGFP)#Ltku |
Information from provider
| Provider | Pawel Pelczar |
| Provider affiliation | Center for Transgenic Models, University of Basel |
| Genetic information | Strain carries a transgene expressing a protamine 1-EGFP (Prm1-EGFP) fusion protein under the transcriptional and translational regulatory control of Prm1. This line shows strong transgene expression. Males are sterile but the ability to mate and to produce copulation plugs is unaffected. Sterility is due to impaired spermatid maturation affecting sperm viability and motility. As such, the males can be used to produce pseudo-pregnant females. See Haueter et al., 2010, Genesis, 48 (3):151-160. |
| Phenotypic information | Homozygous:Mice cannot be bred to homozygosity. Heterozygous:Heterozygous females are phenotypically normal. Heterozygous males are sterile despite normal mating performance. |
| Breeding history | Currently maintained on a CD1 background. |
| References |
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| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Genetic vasectomy-overexpression of Prm1-EGFP fusion protein in elongating spermatids causes dominant male sterility in mice.;Haueter Sabine, Kawasumi Miyuri, Asner Igor, Brykczynska Urszula, Cinelli Paolo, Moisyadi Stefan, Bürki Kurt, Peters Antoine H F M, Pelczar Pawel, ;2010;Genesis (New York, N.Y. : 2000);48;151-60; 20095053
- Circulating miRNAs in Small Extracellular Vesicles Secreted by a Human Melanoma Xenograft in Mouse Brains.;Guglielmi Loredana, Nardella Marta, Musa Carla, Cifola Ingrid, Porru Manuela, Cardinali Beatrice, Iannetti Ilaria, Di Pietro Chiara, Bolasco Giulia, Palmieri Valentina, Vilardo Laura, Panini Nicolò, Bonaventura Fabrizio, Papi Massimiliano, Scavizzi Ferdinando, Raspa Marcello, Leonetti Carlo, Falcone Germana, Felsani Armando, D'Agnano Igea, ;2020;Cancers;12;629; 32575666
- Graphene Oxide Improves in vitro Fertilization in Mice With No Impact on Embryo Development and Preserves the Membrane Microdomains Architecture.;Bernabò Nicola, Valbonetti Luca, Raspa Marcello, Fontana Antonella, Palestini Paola, Botto Laura, Paoletti Renata, Fray Martin, Allen Susan, Machado-Simoes Juliana, Ramal-Sanchez Marina, Pilato Serena, Scavizzi Ferdinando, Barboni Barbara, ;2020;Frontiers in bioengineering and biotechnology;8;; 32612987