B6;129S4-Acantm1c(EUCOMM)Hmgu/Kctt

Status

Available to order

EMMA IDEM:12392
International strain nameB6;129S4-Acantm1c(EUCOMM)Hmgu/Kctt
Alternative nameB6(Cg)-ACANtm1c(EUCOMM)Hmgu/Jwfa
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolAcantm1c(EUCOMM)Hmgu
Gene/Transgene symbolAcan

Information from provider

ProviderAne Charlotte Christensen
Provider affiliationDepartment of Biosciences, University of Oslo
Additional ownerThe ES cells are originally derived from EUCOMM. The derived mouse model is developed in collaboration as described in Rowlands et al. 2018. All parties agree to submission to repository.
Genetic informationTwo JM8A3.N1 embryonic stem (ES) cell lines of agouti C57BL/6N origin (#HFP0602_5_G11 and #HFP0602_5_F12), carrying the targeted Acan gene were purchased from the European Mouse Mutant Cell Repository (EuMMCR). The two ES cell lines are clones containing the same transgenic cassette surrounding exon 4 of the mouse Acan gene, a general “knock-out first” design used by the EUCOMM project, termed targeted allele tm1a (Skarnes et al., 2011). ES cells were plated and prepared according to the supplier’s guidelines before microinjection into 50 blastocysts of albino C57BL/6J Tyr-/- origin and subsequent transfer to 3 pseudo-pregnant females per line. The chimeric offspring, hybrid F1 generation, was selected by appearance of coat color spots originating from transgenic cells. The F1 animals were then crossed with albino C57BL/6J Tyr-/- and transmission of genotype into G1 generation was detected by coat color and verified by PCR genotyping. The mouse was further crossed with a Gt(ROSA)26Sor-FLPe strain expressing flp recombinase, removing the majority of the transgenic cassette and leading to offspring carrying the cre-lox conditional knock-out allele tm1c of Acan, designated as B6(Cg)-Acantm1c(EUCOMM)Hmgu/Jwfa. The tm1c allele is viable as a homozygote. Conditional knock-out of Acan is achieved by removing exon 4 of the Acan gene by cre-lox recombination resulting in the tm1d allele. Loss of exon 4 induces a shift in the reading frame, and thereby prevents protein translation.
Phenotypic informationHomozygous:
Viable, normal phenotype

Heterozygous:
Viable, normal phenotype
Breeding historyC57BL/6N-Atm1Brd ES cells bearing Acantm1a(EUCOMM)Hmgu were used to produce chimeric mice that were then crossed with B6(Cg)-Tyrc-2J/J mice (per JAX Mice, on a C57BL/6J background), whose offspring were crossed to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J or 129S4/SvJaeSor-Gt(ROSA)26Sortm2(FLP*)Sor/J mice and later to B6.Cg-Tg(Nes-cre)1Kln/J (N10 to C57BL/6J) and one cross each to C57BL/6J and C57BL/6JR. The final strain is ~90% C57BL/6J, ~3% C57BL/6N, and ~6% 129S4/SvJaeSor.
References
  • Aggrecan Directs Extracellular Matrix-Mediated Neuronal Plasticity.;Rowlands Daire, Lensjø Kristian K, Dinh Tovy, Yang Sujeong, Andrews Melissa R, Hafting Torkel, Fyhn Marianne, Fawcett James W, Dick Gunnar, ;2018;The Journal of neuroscience : the official journal of the Society for Neuroscience;38;10102-10113; 30282728
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreKarolinska Institutet, Stockholm, Sweden

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • increased circulating HDL cholesterol level / IMPC
  • preweaning lethality, complete penetrance / IMPC
MGI phenotypes (gene matching)
  • abnormal organ of Corti morphology / MGI
  • cleft palate / MGI
  • abnormal trabecular bone morphology / MGI
  • abnormal vertebrae morphology / MGI
  • abnormal vertebral body morphology / MGI
  • abnormal rib morphology / MGI
  • kyphosis / MGI
  • lordosis / MGI
  • abnormal cartilage development / MGI
  • abnormal chondrocyte morphology / MGI
  • short snout / MGI
  • flattened snout / MGI
  • short limbs / MGI
  • abnormal liver morphology / MGI
  • enlarged liver / MGI
  • abnormal spinal cord morphology / MGI
  • abnormal lung morphology / MGI
  • pulmonary hypoplasia / MGI
  • obese / MGI
  • distended abdomen / MGI
  • abnormal gait / MGI
  • aphagia / MGI
  • cardiac hypertrophy / MGI
  • respiratory failure / MGI
  • respiratory distress / MGI
  • deafness / MGI
  • premature death / MGI
  • abnormal limb morphology / MGI
  • abnormal craniofacial bone morphology / MGI
  • no abnormal phenotype detected / MGI
  • disproportionate dwarf / MGI
  • brachydactyly / MGI
  • brachypodia / MGI
  • abnormal joint morphology / MGI
  • short femur / MGI
  • abnormal tracheal cartilage morphology / MGI
  • decreased susceptibility to induced arthritis / MGI
  • polyhydramnios / MGI
  • abnormal intervertebral disk morphology / MGI
  • short humerus / MGI
  • abnormal cochlear inner hair cell morphology / MGI
  • absent cochlear outer hair cells / MGI
  • absent caudal vertebrae / MGI
  • intervertebral disk degeneration / MGI
  • decreased length of long bones / MGI
  • short vertebral column / MGI
  • small thoracic cavity / MGI
  • abnormal cochlear IHC afferent innervation pattern / MGI
  • cardiomyopathy / MGI
  • abnormal limb development / MGI
  • protruding tongue / MGI
  • neonatal lethality, complete penetrance / MGI
  • increased or absent threshold for auditory brainstem response / MGI
  • short basicranium / MGI

Literature references

  • Aggrecan Directs Extracellular Matrix-Mediated Neuronal Plasticity.;Rowlands Daire, Lensjø Kristian K, Dinh Tovy, Yang Sujeong, Andrews Melissa R, Hafting Torkel, Fyhn Marianne, Fawcett James W, Dick Gunnar, ;2018;The Journal of neuroscience : the official journal of the Society for Neuroscience;38;10102-10113; 30282728

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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