B6.129P2(Cg)-Tnfrsf1a^tm3.3Gkl/Flmg

Status	Available to order
EMMA ID	EM:11019
Citation information	RRID:IMSR_EM:11019 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.129P2(Cg)-Tnfrsf1a^tm3.3Gkl/Flmg
Alternative name	B6.129P2(Cg)-Tnfrsf1a/Flmg
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Tnfrsf1a^tm3.3Gkl
Gene/Transgene symbol	Tnfrsf1a

Information from provider

Provider	George Kollias
Provider affiliation	BSRC Fleming
Genetic information	An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted upstream of exon 2. A second loxP site was inserted downstream of exon 5. Flp-mediated recombination removed the selection cassette and left exons 2 through 5 floxed. Cre-mediated recombination, specific to the myeloid lineage, removed exons 2 through 5.
Phenotypic information	Homozygous: No overt phenotype Heterozygous: No overt phenotype
References	Safe TNF-based antitumor therapy following p55TNFR reduction in intestinal epithelium.;Van Hauwermeiren Filip, Armaka Marietta, Karagianni Niki, Kranidioti Ksanthi, Vandenbroucke Roosmarijn E, Loges Sonja, Van Roy Maarten, Staelens Jan, Puimège Leen, Palagani Ajay, Berghe Wim Vanden, Victoratos Panayiotis, Carmeliet Peter, Libert Claude, Kollias George, ;2013;The Journal of clinical investigation;123;2590-603; 23676465 Opposing role of tumor necrosis factor receptor 1 signaling in T cell-mediated hepatitis and bacterial infection in mice.;Wroblewski Raluca, Armaka Marietta, Kondylis Vangelis, Pasparakis Manolis, Walczak Henning, Mittrücker Hans-Willi, Schramm Christoph, Lohse Ansgar W, Kollias George, Ehlken Hanno, ;2016;Hepatology (Baltimore, Md.);64;508-21; 26991125
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	B.S.R.C. Alexander Fleming, Vari, Greece
Animals used for archiving	heterozygous C57BL/6 males

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Tumor necrosis factor receptor 1 associated periodic syndrome / Orphanet_32960

IMPC phenotypes (gene matching)

abnormal skin morphology / IMPC
increased large unstained cell number / IMPC
increased grip strength / IMPC
increased basophil cell number / IMPC
increased neutrophil cell number / IMPC
increased eosinophil cell number / IMPC
increased NK cell number / IMPC
abnormal cholesterol homeostasis / IMPC
decreased circulating calcium level / IMPC
increased lymphocyte cell number / IMPC
increased effector memory CD8-positive, alpha-beta T cell number / IMPC
increased circulating aspartate transaminase level / IMPC
enlarged heart / IMPC
increased leukocyte cell number / IMPC
decreased monocyte cell number / IMPC
abnormal heart morphology / IMPC
decreased neutrophil cell number / IMPC

MGI phenotypes (gene matching)

increased bone mineral density / MGI
abnormal Peyer's patch morphology / MGI
abnormal sleep pattern / MGI
abnormal immune system physiology / MGI
decreased IgG level / MGI
liver inflammation / MGI
lung inflammation / MGI
decreased inflammatory response / MGI
increased skin papilloma incidence / MGI
decreased tumor incidence / MGI
abnormal respiratory mechanics / MGI
abnormal airway responsiveness / MGI
abnormal lymph node B cell domain morphology / MGI
abnormal spleen germinal center morphology / MGI
abnormal Peyer's patch follicle morphology / MGI
decreased susceptibility to bacterial infection / MGI
increased susceptibility to bacterial infection / MGI
abnormal macrophage physiology / MGI
granulomatous inflammation / MGI
impaired skin barrier function / MGI
abnormal glutamate-mediated receptor currents / MGI
decreased circulating alanine transaminase level / MGI
abnormal cytokine secretion / MGI
no phenotypic analysis / MGI
abnormal nervous system physiology / MGI
abnormal response/metabolism to endogenous compounds / MGI
increased susceptibility to experimental autoimmune encephalomyelitis / MGI
decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
decreased susceptibility to autoimmune diabetes / MGI
increased susceptibility to parasitic infection / MGI
increased susceptibility to type I hypersensitivity reaction / MGI
decreased Peyer's patch number / MGI
abnormal follicular dendritic cell morphology / MGI
absent follicular dendritic cells / MGI
decreased IgG1 level / MGI
increased circulating tumor necrosis factor level / MGI
decreased circulating interleukin-6 level / MGI
decreased circulating interleukin-1 beta level / MGI
increased interleukin-6 secretion / MGI
decreased interleukin-6 secretion / MGI
abnormal chemokine secretion / MGI
decreased susceptibility to endotoxin shock / MGI
increased susceptibility to endotoxin shock / MGI
decreased physiological sensitivity to xenobiotic / MGI
increased sensitivity to induced cell death / MGI
increased sensitivity to induced morbidity/mortality / MGI
decreased sensitivity to induced morbidity/mortality / MGI
decreased sensitivity to xenobiotic induced morbidity/mortality / MGI
increased susceptibility to bacterial infection induced morbidity/mortality / MGI
abnormal neuron proliferation / MGI
abnormal oval cell physiology / MGI
tumor regression / MGI
impaired adaptive thermogenesis / MGI
decreased susceptibility to dopaminergic neuron neurotoxicity / MGI
decreased microglial cell number / MGI
impaired humoral immune response / MGI
impaired central nervous system regeneration / MGI

Literature references

Safe TNF-based antitumor therapy following p55TNFR reduction in intestinal epithelium.;Van Hauwermeiren Filip, Armaka Marietta, Karagianni Niki, Kranidioti Ksanthi, Vandenbroucke Roosmarijn E, Loges Sonja, Van Roy Maarten, Staelens Jan, Puimège Leen, Palagani Ajay, Berghe Wim Vanden, Victoratos Panayiotis, Carmeliet Peter, Libert Claude, Kollias George, ;2013;The Journal of clinical investigation;123;2590-603; 23676465
Opposing role of tumor necrosis factor receptor 1 signaling in T cell-mediated hepatitis and bacterial infection in mice.;Wroblewski Raluca, Armaka Marietta, Kondylis Vangelis, Pasparakis Manolis, Walczak Henning, Mittrücker Hans-Willi, Schramm Christoph, Lohse Ansgar W, Kollias George, Ehlken Hanno, ;2016;Hepatology (Baltimore, Md.);64;508-21; 26991125

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6.129P2(Cg)-Tnfrsf1atm3.3Gkl/Flmg