STOCK Pik3cgtm1a(EUCOMM)Wtsi/WtsiOulu

Status

Available to order

EMMA IDEM:05431
Citation informationRRID:IMSR_EM:05431 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameSTOCK Pik3cgtm1a(EUCOMM)Wtsi/WtsiOulu
Alternative nameEPD0359_4_A06
Strain typeTargeted Mutant Strains
Allele/Transgene symbolPik3cgtm1a(EUCOMM)Wtsi
Gene/Transgene symbolPik3cg
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0359_4_A06. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreUniversity of Oulu, Oulu, Finland

Disease and phenotype information

IMPC phenotypes (allele matching)
  • decreased circulating potassium level / IMPC
IMPC phenotypes (gene matching)
  • decreased circulating potassium level / IMPC
MGI phenotypes (allele matching)
  • decreased circulating alanine transaminase level / MGI
MGI phenotypes (gene matching)
  • increased leukocyte cell number / MGI
  • increased neutrophil cell number / MGI
  • increased monocyte cell number / MGI
  • decreased neutrophil cell number / MGI
  • abnormal myocardial fiber morphology / MGI
  • decreased thymocyte number / MGI
  • skin lesions / MGI
  • weight loss / MGI
  • abnormal immune cell physiology / MGI
  • thymus hypoplasia / MGI
  • abnormal T cell activation / MGI
  • abnormal inflammatory response / MGI
  • decreased inflammatory response / MGI
  • abnormal definitive hematopoiesis / MGI
  • abnormal T cell differentiation / MGI
  • increased susceptibility to bacterial infection / MGI
  • increased susceptibility to viral infection / MGI
  • abnormal leukocyte physiology / MGI
  • abnormal T cell physiology / MGI
  • abnormal macrophage physiology / MGI
  • decreased immunoglobulin level / MGI
  • abnormal neutrophil physiology / MGI
  • abnormal blood coagulation / MGI
  • decreased circulating alanine transaminase level / MGI
  • decreased susceptibility to induced arthritis / MGI
  • impaired macrophage chemotaxis / MGI
  • decreased macrophage cell number / MGI
  • abnormal cardiac muscle relaxation / MGI
  • abnormal CD8-positive, alpha-beta T cell physiology / MGI
  • decreased platelet calcium level / MGI
  • increased eosinophil cell number / MGI
  • abnormal response to infection / MGI
  • decreased cytotoxic T cell cytolysis / MGI
  • decreased T cell proliferation / MGI
  • abnormal heart ventricle morphology / MGI
  • immune system phenotype / MGI
  • abnormal platelet physiology / MGI
  • abnormal T-helper 2 physiology / MGI
  • increased cardiac muscle contractility / MGI
  • abnormal NK cell differentiation / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • increased tumor necrosis factor secretion / MGI
  • decreased interferon-gamma secretion / MGI
  • increased interleukin-1 beta secretion / MGI
  • decreased interleukin-2 secretion / MGI
  • increased interleukin-6 secretion / MGI
  • impaired neutrophil recruitment / MGI
  • impaired neutrophil chemotaxis / MGI
  • decreased platelet aggregation / MGI
  • increased sensitivity to induced morbidity/mortality / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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