Thawing Protocol for GEMM ESC Clones
Validated clones ready for ordering
Clone Id | Clone description |
|---|---|
JJ7575 | Embryonic stem cells derived from a K14-Cre;Brca1F/F;Trp53F/F mouse (FVB/N background). Clone A2. Official strain nomenclature: FVB(129).Cg-Trp53tm1BrnBrca1tm1BrnTg(KRT14-cre)8Brn/A. |
JJ7334 | Embryonic stem cells derived from a K14-Cre;Brca1F/F;Trp53F/F mouse (FVB/N background). Clone C7. Official strain nomenclature: FVB(129).Cg-Trp53tm1BrnBrca1tm1BrnTg(KRT14-cre)8Brn/A. |
JJ8160 | Embryonic stem cells derived from a K14-Cre;Brca1F/F;Trp53F/F mouse (FVB/N background) targeted with the Col1a1-frt vector allowing Flp-recombinase mediated integration of a transgene-coding plasmid in the Col1a1 locus (Beard et al., 2006). |
JJ9965 | Embryonic stem cells derived from a WAP-Cre;Brca1F/F;Trp53F/F mouse (FVB/N background). |
IH20096 | Embryonic stem cells derived from a WAP-Cre;Brca1F/F;Trp53F/F mouse (FVB/N background) targeted with the Col1a1-frt vector allowing Flp-recombinase mediated integration of a transgene-coding plasmid in the Col1a1 locus (Beard et al., 2006). |
JJ8059 | Embryonic stem cells derived from a WAP-Cre;Trp53F/F mouse (FVB/N background). |
JJ9507 | Embryonic stem cells derived from a WAP-Cre;Trp53F/F mouse (FVB/N background) targeted with the Col1a1-frt vector allowing Flp-recombinase mediated integration of a transgene-coding plasmid in the Col1a1 locus (Beard et al., 2006). |
JJ7373 | Embryonic stem cells derived from a WAP-Cre;Cdh1F/F mouse (FVB/N background). |
JJ8641 | Embryonic stem cells derived from a WAP-Cre;Cdh1F/F mouse (FVB/N background). |
IHM20047 | Embryonic stem cells derived from a WAP-Cre;Cdh1F/F mouse (FVB/N background) targeted with the Col1a1-frt vector allowing Flp-recombinase mediated integration of a transgene-coding plasmid in the Col1a1 locus (Beard et al., 2006). |
TMA11473 | Embryonic stem cells derived from a KrasLSL-G12D/+ mouse (C57BL/6J background); clone 2.7. Official strain nomenclature: B6.129S4-Krastm4Tyj/JA. Reference: Huijbers et al., 2014 (PMID: 24401838). |
TMA10114 | Embryonic stem cells derived from a Rb1F/F;P53F/F mouse (FVB/N;129/Ola mixed background with white coat color); clone 1.5. Official strain nomenclature: FVB;129P2-Trp53tm1Brn Rb1tm1Brn/A. Reference: Huijbers et al., 2014 (PMID: 24401838). |
TMA10132 | Embryonic stem cells derived from a Rb1F/F;P53F/F mouse (FVB/N;129/Ola mixed background with white coat color); clone 1.10. Official strain nomenclature: FVB;129P2-Trp53tm1Brn Rb1tm1Brn/A. Reference: Huijbers et al., 2014 (PMID: 24401838). |
TMA10135 | Embryonic stem cells derived a Nf2F/F;Trp53F/F;Cdkn2a/ mouse (FVB/N;129/Ola mixed background with grey/agouti coat color); clone 1.1. Official strain nomenclature: FVB;129P2-Cdkn2atm1.1Brn Nf2tm2Gth Trp53tm1Brn. Huijbers et al., 2014 (PMID: 24401838). |
TMA10106 | Embryonic stem cells derived from a Nf2F/F;Trp53F/F;Cdkn2a/ mouse (FVB/N;129/Ola mixed background with grey/agouti coat color); clone 1.5. Official strain nomenclature: FVB;129P2-Cdkn2atm1.1Brn Nf2tm2Gth Trp53tm1Brn. Huijbers et al., 2014 (PMID: 24401838). |
IH12059 | Embryonic stem cells derived from a KrasLSL-G12D/+ mouse (C57BL/6J background) targeted with the Col1a1-frt vector allowing Flp-recombinase mediated integration of a transgene-coding plasmid in the Col1a1 locus (Beard et al., 2006); clone 2.7_1. Official strain nomenclature: B6.Cg-Krastm4Tyj Col1a1tm1Nki/A. Reference: Huijbers et al., 2014 (PMID: 24401838). |
TMA10819 | Embryonic stem cells derived from a KrasLSL-G12D/+ mouse (C57BL/6J background) targeted with the Col1a1-frt vector allowing Flp-recombinase mediated integration of a transgene-coding plasmid in the Col1a1 locus (Beard et al., 2006); clone 2.7_45. Official strain nomenclature: B6.Cg-Krastm4Tyj Col1a1tm1Nki/A. Reference: Huijbers et al., 2014 (PMID: 24401838). |
IH12060 | Embryonic stem cells derived from a KrasLSL-G12D/+ mouse (C57BL/6J background) targeted with the Col1a1-frt vector allowing Flp-recombinase mediated integration of a transgene-coding plasmid in the Col1a1 locus (Beard et al., 2006); clone 2.7_64. Official strain nomenclature: B6.Cg-Krastm4Tyj Col1a1tm1Nki/A. Reference: Huijbers et al., 2014 (PMID: 24401838). |
TMA10873 | Embryonic stem cells derived from a KrasLSL-G12D/+ mouse (C57BL/6J background) targeted with the Col1a1-frt vector allowing Flp-recombinase mediated integration of a transgene-coding plasmid in the Col1a1 locus (Beard et al., 2006); clone 2.7_66. Official strain nomenclature: B6.Cg-Krastm4Tyj Col1a1tm1Nki/A. Reference: Huijbers et al., 2014 (PMID: 24401838). |
TMA10353 | Embryonic stem cells derived from a Rb1F/F;P53F/F mouse (FVB/N;129/Ola mixed background with white coat color) targeted with the Col1a1-frt vector allowing Flp-recombinase mediated integration of a transgene-coding plasmid in the Col1a1 locus (Beard et al., 2006); clone 1.5_1A10. Official strain nomenclature: FVB;129P2-Trp53tm1Brn Col1a1tm2Nki Rb1tm2Brn/A. Reference: Huijbers et al., 2014 (PMID: 24401838). |
TMA11101 | Embryonic stem cells derived from a Col1a1-frt;Rb1F/F;P53F/F mouse (FVB/N;129/Ola mixed background with white coat color) suitable for Flp-recombinase mediated integration of a transgene-coding plasmid in the Col1a1 locus (Beard et al., 2006); clone 1.5_1B1_r4. Official strain nomenclature: FVB;129P2-Trp53tm1Brn Col1a1tm2Nki Rb1tm2Brn/A. Reference: Huijbers et al., 2014 (PMID: 24401838). |
IH12076 | Embryonic stem cells derived from a Nf2F/F;Trp53F/F;Cdkn2a/ mouse (FVB/N;129/Ola mixed background with grey/agouti coat color) targeted with the Col1a1-frt vector allowing Flp-recombinase mediated integration of a transgene-coding plasmid in the Col1a1 locus (Beard et al., 2006); clone 1.1_1E5. Official strain nomenclature: FVB;129P2-Cdkn2atm1.1Brn Nf2tm2Gth Trp53tm1Brn Col1a1tm3Nki/A. Huijbers et al., 2014 (PMID: 24401838). |
TMA10668 | Embryonic stem cells derived from a Nf2F/F;Trp53F/F;Cdkn2a/ mouse (FVB/N;129/Ola mixed background with grey/agouti coat color) targeted with the Col1a1-frt vector allowing Flp-recombinase mediated integration of a transgene-coding plasmid in the Col1a1 locus (Beard et al., 2006); clone 1.1_1F6_s6. Official strain nomenclature: FVB;129P2-Cdkn2atm1.1Brn Nf2tm2Gth Trp53tm1Brn Col1a1tm3Nki/A. Huijbers et al., 2014 (PMID: 24401838). |
Protocols and maps
Protocols
Maps
References
Methodology and concept:
Cell Rep. 2016 Jul 19. Transcription Factor NFIB Is a Driver of Small Cell Lung Cancer Progression in Mice and Marks Metastatic Disease in Patients. Semenova EA, Kwon MC, Monkhorst K, Song JY, Bhaskaran R, Krijgsman O, Kuilman T, Peters D, Buikhuisen WA, Smit EF, Pritchard C, Cozijnsen M, van der Vliet J, Zevenhoven J, Lambooij JP, Proost N, van Montfort E, Velds A, Huijbers IJ, Berns A.
Genes Dev. 2016 Jun 15. Modeling invasive lobular breast carcinoma by CRISPR/Cas9-mediated somatic genome editing of the mammary gland. Annunziato S, Kas SM, Nethe M, Yücel H, Del Bravo J, Pritchard C, Bin Ali R, van Gerwen B, Siteur B, Drenth AP, Schut E, van de Ven M, Boelens MC, Klarenbeek S, Huijbers IJ, van Miltenburg MH, Jonkers J.
Nature Protocols 2015 Oct 15. Using the GEMM-ESC strategy to study gene function in mouse models. Huijbers IJ, Del Bravo J, Bin Ali R, Pritchard C, Braumuller TM, van Miltenburg MH, Henneman L, Michalak EM, Berns A & Jonkers J.
PNAS. 2015 Jun 22. Selective resistance to the PARP inhibitor olaparib in a mouse model for BRCA1-deficient metaplastic breast cancer. Henneman L, van Miltenburg MH, Michalak EM, Braumuller TM, Jaspers JE, Drenth AP, Grimmerink R, Gogola E, Szuhai K, Schlicker A, Bin Ali R, Pritchard C, Huijbers IJ, Berns A, Rottenberg S, Jonkers J.
EMBO Mol Med. 2014 Feb;6(2):212-25. Rapid target gene validation in complex cancer mouse models using re-derived embryonic stem cells. Huijbers IJ, Bin Ali R, Pritchard C, Cozijnsen M, Kwon MC, Proost N, Song JY, de Vries H, Badhai J, Sutherland K, Krimpenfort P, Michalak EM, Jonkers J, Berns A.
Curr Opin Genet Dev. 2012 Feb;22(1):21-7. doi: 10.1016/j.gde.2012.01.004. Epub 2012 Feb 8. Using genetically engineered mouse models to validate candidate cancer genes and test new therapeutic approaches. van Miltenburg MH, Jonkers J.
Bioessays. 2011 Sep;33(9):701-10. doi: 10.1002/bies.201100018. Epub 2011 Jul 7. Rapid validation of cancer genes in chimeras derived from established genetically engineered mouse models. Huijbers IJ, Krimpenfort P, Berns A, Jonkers J.
GEMM development:
Cancer Cell. 2003 Sep;4(3):181-9. Induction of small cell lung cancer by somatic inactivation of both Trp53 and Rb1 in a conditional mouse model. Meuwissen R, Linn SC, Linnoila RI, Zevenhoven J, Mooi WJ, Berns A. See clone id: TMA10114, TMA10132, TMA10353, TMA11101.
Cancer Cell. 2004 Apr;5(4):375-87. Endogenous oncogenic K-ras(G12D) stimulates proliferation and widespread neoplastic and developmental defects. Tuveson DA, Shaw AT, Willis NA, Silver DP, Jackson EL, Chang S, Mercer KL, Grochow R, Hock H, Crowley D, Hingorani SR, Zaks T, King C, Jacobetz MA, Wang L, Bronson RT, Orkin SH, DePinho RA, Jacks T. See clone id: TMA11473, IH12059, TMA10819, IH12060, TMA10873
Cancer Cell. 2008 Mar;13(3):261-71. A conditional mouse model for malignant mesothelioma. Jongsma J, van Montfort E, Vooijs M, Zevenhoven J, Krimpenfort P, van der Valk M, van de Vijver M, Berns A. See clone id: TMA10135, TMA10106, IH12076, TMA10668
Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):12111-6. Somatic loss of BRCA1 and p53 in mice induces mammary tumors with features of human BRCA1-mutated basal-like breast cancer. Liu X, Holstege H, van der Gulden H, Treur-Mulder M, Zevenhoven J, Velds A, Kerkhoven RM, van Vliet MH, Wessels LF, Peterse JL, Berns A, Jonkers J. See clone id: JJ7575, JJ7334, JJ8160
Cancer Cell. 2006 Nov;10(5):437-49. Somatic inactivation of E-cadherin and p53 in mice leads to metastatic lobular mammary carcinoma through induction of anoikis resistance and angiogenesis. Derksen PW, Liu X, Saridin F, van der Gulden H, Zevenhoven J, Evers B, van Beijnum JR, Griffioen AW, Vink J, Krimpenfort P, Peterse JL, Cardiff RD, Berns A, Jonkers J. See clone id: JJ9965, IH20096, JJ8059, JJ9507, JJ7373, JJ8641, IHM20047