COVID-19 Resources and Services

To combat the rapidly evolving COVID-19 pandemic, INFRAFRONTIER is offering, free-of-charge open calls, several resources, COVID-19-related strains and emergency archiving services to the global research community with the aim to aid COVID-19 research and minimise the loss of valuable mouse strains during the crisis.

With more than 1.5 million deaths by the end of 2020 and a long-lasting presence, the COVID-19 pandemic is one of the deadliest diseases to plague humankind in recent times. Considerable scientific effort from all over the world has been rallied to fight this common foe.

COVID-19 Open Calls

These are specific free-of-charge calls for project proposals funded by prominent third-parties like the European Commission or National Ministries of our partners. Users can submit their COVID-19 project proposals to utilise cutting-edge services provided by INFRAFRONTIER partners.

COVID-19 BSL3 Pipeline

This ISIDORe Open Call provides extensive user support to the selected projects for assessing novel potential vaccine and treatment compounds for COVID-19 in preclinical mouse models.

Coronaviridae-related EMMA strains

The following EMMA strains are related to SARS-CoV-2 and other Coronaviridae viral infections via their human orthologues. The table below contains EMMA strains that could be used for Coronaviridae infection research.

Gene
EMMA Strain Details
Available Resources
Adam17

EM:04475 / CNB-CSIC

additional information

CNB-CSIC
Lluis Montoliu

frozen germplasm

TMPRSS2 and ADAM17 Cleave ACE2 Differentially and Only Proteolysis by TMPRSS2 Augments Entry Driven by the Severe Acute Respiratory Syndrome Coronavirus Spike Protein

Zdhhc5

EM:11531 / HMGU

additional information

HMGU
Susan Marschall

frozen germplasm

EM:05839 / CNR

additional information

CNR
Marcello Raspa

frozen germplasm

A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Repurposing

‘the Spike protein (S) interacts with the GOLGA7-ZDHHC5 acyl-transferase complex, which likely mediates palmitoylation on its cytosolic tail’

Trmt1

EM:10456 / ICS

additional information

ICS
Mohammed Selloum

frozen germplasm

A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Repurposing

‘We also identified an interaction of Nsp5 (C145A) with tRNA methyltransferase 1 (TRMT1), which is responsible for synthesis of the dimethylguanosine (m2,2G) base modification on both nuclear and mitochondrial tRNAs40. We predict TRMT1 is also cleaved by Nsp5, removing its zinc finger and nuclear localization signal and likely resulting in an exclusively mitochondrial localization’

Bsg

EM:04318 / CNRS

additional information

CNRS
Karine Jambou

frozen germplasm

CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells

‘Here, we reported a research finding that SARS-CoV-2 invaded host cells via a novel route of CD147-spike protein (SP). SP bound to CD147, a receptor on the host cells, thereby mediating the viral invasion.’

Dhodh

EM:06847 / Vetmeduni Vienna

additional information

Vetmeduni Vienna
Auke Boersma

frozen germplasm

EM:14341 / MRC Harwell Institute

additional information

MRC Harwell Institute
Martin Fray

frozen germplasm

Parallel shRNA and CRISPR-Cas9 screens enable antiviral drug target identification

Os9

EM:05527 / University of Oulu

additional information

University of Oulu
Reetta Hinttala

frozen germplasm

A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Repurposing

Rab5c

EM:07127 / University of Oulu

additional information

University of Oulu
Reetta Hinttala

frozen germplasm

A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Repurposing

Tmprss2

EM:09886 / CNRS

additional information

CNRS
Karine Jambou

frozen germplasm

SARS‐CoV‐2 receptor ACE2 and TMPRSS2 are primarily expressed in bronchial transient secretory cells

Retreg3

EM:05438 / University of Oulu

additional information

University of Oulu Reetta Hinttala

frozen germplasm

A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Repurposing

Dpp4

EM:02577 / CNRS

additional information

CNRS
Karine Jambou

frozen germplasm

EM:09968 / CNRS

additional information

CNRS
Karine Jambou

frozen germplasm

COVID-19 and diabetes: Can DPP4 inhibition play a role?

Mouse-adapted MERS coronavirus causes lethal lung disease in human DPP4 knockin mice

Multi-Organ Damage in Human Dipeptidyl Peptidase 4 Transgenic Mice Infected with Middle East Respiratory Syndrome-Coronavirus

Generation of a Transgenic Mouse Model of Middle East Respiratory Syndrome Coronavirus Infection and Disease

Middle East Respiratory Syndrome Coronavirus Causes Multiple Organ Damage and Lethal Disease in Mice Transgenic for Human Dipeptidyl Peptidase 4

A mouse model for MERS coronavirus-induced acute respiratory distress syndrome

A Human DPP4-Knockin Mouse’s Susceptibility to Infection by Authentic and Pseudotyped MERS-CoV

Acute Respiratory Infection in Human Dipeptidyl Peptidase 4-Transgenic Mice Infected With Middle East Respiratory Syndrome Coronavirus

Furin

EM:04412 / CNR

additional information

CNR
Marcello Raspa

frozen germplasm

The Proximal Origin of SARS-CoV-2

‘the highly variable spike (S) protein of SARS-CoV-2 has a polybasic (furin) cleavage site at the S1 and S2 boundary via the insertion of twelve nucleotides’

Stat1

EM:08708 / CNRS

additional information

CNRS
Karine Jambou

frozen germplasm

EM:09915 / Vetmeduni Vienna

additional information

Vetmeduni Vienna
Auke Boersma

frozen germplasm

EM:09916 / Vetmeduni Vienna

additional information

Vetmeduni Vienna
Auke Boersma

frozen germplasm

EM:09906 / Vetmeduni Vienna

additional information

Vetmeduni Vienna
Auke Boersma

frozen germplasm

EM:06064 / CNB-CSIC

additional information

CNB-CSIC
Lluis Montoliu

frozen germplasm

EM:06065 / CNB-CSIC

additional information

CNB-CSIC
Lluis Montoliu

frozen germplasm

SARS-CoV-2 is sensitive to type I interferon pretreatment

Resolution of Primary Severe Acute Respiratory Syndrome-Associated Coronavirus Infection Requires Stat1

SARS-CoV Pathogenesis Is Regulated by a STAT1 Dependent but a Type I, II and III Interferon Receptor Independent Mechanism

Il6

EM:05332 / CNB-CSIC

additional information

CNB-CSIC
Lluis Montoliu

Detectable serum SARS-CoV-2 viral load (RNAaemia) is closely associated with drastically elevated interleukin 6 (IL-6) level in critically ill COVID-19 patients

Severe Acute Respiratory Syndrome (SARS) Coronavirus-Induced Lung Epithelial Cytokines Exacerbate SARS Pathogenesis by Modulating Intrinsic Functions of Monocyte-Derived Macrophages and Dendritic Cells

Medical Authorities and Companies launch novel clinical trials of interleukin 6 inhibitors, as the IL-6 pathway could play a role in the overactive inflammatory response observed in lungs of severely and critically ill COVID-19 patients http://www.chictr.org.cn/showprojen.aspx?proj=49409 https://www.pmlive.com/pharma_news/sanofi,_regeneron_launch_trials_of_arthritis_drug_kevzara_for_covid-19_1329326

Il10

EM:11812 / CNRS

additional information

CNRS
Karine Jambou

frozen germplasm

EM:08503 / CNB-CSIC

additional information

CNB-CSIC
Lluis Montoliu

EM:08502 / CNB-CSIC

additional information

CNB-CSIC
Lluis Montoliu

Highly Activated Cytotoxic CD8 T Cells Express Protective IL-10 at the Peak of Coronavirus-Induced Encephalitis

Profiling serum cytokines in COVID-19 patients reveals IL-6 and IL-10 are disease severity predictors

Myd88

EM:06164 / HMGU

additional information

HMGU
Susan Marschall

frozen germplasm

EM:05518 / University of Oulu

additional information

University of Oulu
Reetta Hinttala

frozen germplasm

MyD88 Is Required for Protection from Lethal Infection with a Mouse-Adapted SARS-CoV

Nrp

EM:11987 / MRC Harwell Institute

additional information

MRC Harwell Institute
Martin Fray

frozen germplasm

EM:11988 / MRC Harwell Institute

additional information

MRC Harwell Institute
Martin Fray

frozen germplasm

EM:09943 / CNRS

additional information

CNRS
Karine Jambou

frozen germplasm

Neuropilin-1 facilitates SARS-CoV-2 cell entry and provides a possible pathway into the central nervous system

Tlr4

EM:00035 / CNRS

additional information

CNRS
Karine Jambou

frozen germplasm

Identification of Oxidative Stress and Toll-like Receptor 4 Signaling as a Key Pathway of Acute Lung Injury

Ifnar1

EM:10181 / University of Oulu

additional information

University of Oulu
Reetta Hinttala

frozen germplasm

Type I Interferons Are Essential in Controlling Neurotropic Coronavirus Infection Irrespective of Functional CD8 T Cells

Inborn errors of type I IFN immunity in patients with life-threatening COVID-19

Ahr

EM:12733 / Czech Centre for Phenogenomics

additional information

Czech Centre for Phenogenomics

frozen germplasm

Murine Coronavirus Infection Activates the Aryl Hydrocarbon Receptor in an Indoleamine 2,3-Dioxygenase-Independent Manner, Contributing to Cytokine Modulation and Proviral TCDD-Inducible-PARP Expression

Ifih1

EM:11517 / MRC Harwell Institute

additional information

MRC Harwell Institute
Martin Fray

frozen germplasm

Pangolins Lack IFIH1/MDA5, a Cytoplasmic RNA Sensor That Initiates Innate Immune Defense Upon Coronavirus Infection

MDA5 Is Critical to Host Defense during Infection with Murine Coronavirus

CCr1

EM:11132 / Vetmeduni Vienna

additional information

Vetmeduni Vienna
Auke Boersma

frozen germplasm

EM:11144 / Vetmeduni Vienna

additional information

Vetmeduni Vienna
Auke Boersma

frozen germplasm

Phenotypes associated with this genotype from MGI: link

MyD88 is required for protection from lethal infection with a mouse-adapted SARS-CoV

Stat1, Tg(ANPEP)270Mmul

EM:06060 / CNB-CSIC

additional information

CNB-CSIC
Lluis Montoliu

frozen germplasm

EM:06061 / CNB-CSIC

additional information

CNB-CSIC
Lluis Montoliu

frozen germplasm

Phenotypes associated with this genotype from MGI: link

Development of a transgenic mouse model susceptible to human coronavirus 229E

Ifng

EM:02106 / CNR

additional information

CNR
Marcello Raspa

frozen germplasm

Phenotypes associated with this genotype from MGI: link

IFN-gamma is required for viral clearance from central nervous system oligodendroglia

Tbk1

EM:07230 / CNB-CSIC

additional information

CNB-CSIC
Lluis Montoliu

frozen germplasm

EM:12874 / Czech Centre for Phenogenomics

additional information

Czech Centre for Phenogenomics

frozen germplasm

Inborn errors of type I IFN immunity in patients with life-threatening COVID-19

Irf7

EM:14100 / CNRS

additional information

CNRS
Karine Jambou

frozen germplasm

Inborn errors of type I IFN immunity in patients with life-threatening COVID-19

Emergency Archiving during COVID-19 crisis

Due to the current coronavirus (COVID-19) situation, there may be a need to urgently archive various valuable mouse models. Please contact us (emma@infrafrontier.eu) for archiving possibilities. In contrast to the standard EMMA archiving service, which includes evaluation by an external scientific committee and is free of charge for depositors, the emergency service may come with service charges. Details will be provided by the EMMA nodes.

As the situation in the different countries is changing constantly, we can not give any guarantees. Whether mice can be imported also strongly depends on whether carrier companies are still servicing the respective route from the depositor institute to the EMMA node.

COVID-19 resources from our partners and collaborators

Here is a list of COVID-19 research resources offered by our European and international partners:

Mouse Genome Informatics Coronavirus Information Center
Life Science RI COVID-19 response
EOSC-Life: LS RI Responses to COVID-19
ESFRI COVID-19 Services & Resources
Research Infrastructures and COVID-19 Research from ERF-AISBL
The Global Mouse Models for COVID-19 Consortium

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